垂体腺瘤细胞系建立及相关性分析-establishment and correlation analysis of pituitary adenoma cell line.docxVIP

Abstractof nucleolus and hypertrophy of mitochondria with sparely excreted granules.6 、 Tt showed an epithelial -like morphology with large cell nuclei and abundant cytoplasm.Electron microscopy of culture cell revealed that free cell surfaces were raisedmultiple filopodia ， Nuclei contained margination and enlargement of nucleolus. The cytoplasm contained mitochondrial hypertrophy and sparely granulated.Conclusions:The chromosome of pituitary adenoma with in viro was Karyological characteristics of thechromosome.DNA ploidy analysis shows that is Karyological characteristics for direction to develop.Tt is relation to pituitary adenoma for secretion and proliferation function of pituitary adenoma.Culture in viro of the pituitary adenoma cell lines with neuroendocrine features，whichtumor central area of chromosome DNA might fracture and damage repair enzymes would be to suppress，its expression will to reinforce，Chromosomal Fragile Site has increase to suffer form adenoma for the risk carriers to linker tuomrigenesis4. the immunohistochemistery assay showed that human prolactin protein staining was positive while chromosome of pituitary adenoma has change lead to uncontrolled proliferation of tumor cells and to linker histone modification enzymes cyclin family， the close relationship5.putiutary adenoma in viro with monoclonalKey word pituitary adenomas cell lines ， Karyological characteristics ， non-functioning pituitary adenomas(NFPAs)，monoclonalTV河北大学学位论文独创性声明本人郑重声明 : 所呈交的学位论文，是本人在导师指导下进行的研究工作及取得 的研究成果 。尽我所知 ， 除了文巾特别加以标注和致谢的地方外 ，论文中不包含其他 人已经发表或撰写的研究成果，也不包含为获得河北大学或其他教育机构的学位或证书 所使用过的材料。与我 一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示了致谢 。作者签名 : 日期: .ko1上年j一月二_R学位论文使用授权声明本人完全了解河北大学有关保留、使用学位论文的规定，即 :学校有权保留并向国 家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。学校可以公布论文的全部或部分内容 ，可以采用影印、缩印或其他复制手段保存论文 。本学位论文属于1、保密口，在 年一一一月一一_R 解密后适用本授权声明 。2、不保密。〈请在以上相应方格内打 .J )保护知识产权声明本人为申请河北大学学位所提交的题目为蚓切地 t. i(fA ft很的学位 论文，是我个人在导师任沁指导并与导师合作下取得的研究成果，研究工作及取得的研究成果是在河北大学所提供的研究经费及导师的研究经费资助下完成的