食管鳞癌TGFβ受体ⅠⅡ基因启动子区甲基化状态的研究.pptVIP

前 言 研究目的 本研究应用甲基化特异性PCR反应(MSP)和RT-PCR技术检测食管鳞状细胞癌组织中TGF-β受体Ⅰ、Ⅱ基因(TβRⅠ、Ⅱ)启动子区甲基化状态和mRNA表达水平，并结合临床资料探讨其与临床病理资料的关系，为确定TβRⅠ、Ⅱ甲基化与ESCC发生发展之间的关系提供理论依据。 Methylation analysis of TβRⅠand TβRⅡ in tumor tissue (T) and corresponding normal tissues (N). u: indicates the presence of unmethylated genes; m: indicates the presence of methylated genes. Cases 1: tumor-specific methylation; Case 2: the tumor is fully methylated, whereas the corresponding normal tissue has a very faint band demonstrating methylation; Case 3: both of tumor and corresponding normal tissue unm