温度和培养基在克孢子丝菌酵母相转化中的作用.docVIP

使用前删除此我爱浪漫樱花文档主页： HYPERLINK /mtl168168 /mtl168168，分享文档快乐生活快乐无阻PAGE PAGE 5浅谈???????????? 作者：张静， 黄怀球， 薛汝增 张晓辉 冯佩英 李美荣【摘要】? 【目的】 探讨不同温度和培养基在申克孢子丝菌酵母相转化中的作用。【方法】 将4株临床分离菌株分别接种于sda、pda、bhi、50 mg/kg羊血bhi及5 mg/kg gsbhi 5种培养基平皿上，每种培养基接种5个平皿，分别置于25 ℃、35 ℃、36 ℃、37 ℃及38 ℃含体积百分数为5%co2的恒温箱中培养。观察菌落生长情况并计算酵母细胞转化率。【结果】 在38 ℃，平皿转化率为25%(只有1株菌发生了转化);在35 ℃和37 ℃时，平皿转化率分别是60%和85%;而在36 ℃时平皿转化率仅为45%。使用50 mg/kg羊血bhi和0.5%gsbhi培养基培养，65%培养皿上的菌株发生了酵母相转化，使用bhi培养基培养，45%培养皿上的菌株发生了酵母相转化，而使用sda和pda培养基培养，只有20%培养皿上的菌株发生了酵母相转化。37 ℃时，有3株菌在bhi、50 mg/kg羊血bhi和5 mg/kg gsbhi培养基上的酵母细胞转化率均达到90%，1株菌在sda、pda和bhi培养基上的转化率均低于10%。【结论】 温度是影响申克孢子丝菌酵母相转化的关键因素，培养基的营养成分亦是影响转化效果的重要因素。 【关键词】? 申克孢子丝菌; 酵母相转化; 温度; 培养基　abstract： 【objective】 to investigate the effect of temperature and media on mycelium-to-yeast transformation of sporothrix schenckii. 【methods】 the clinical isolates 4 were inoculated on plates of media after reactivation. each isolate was inoculated on plates of sda， pda， bhi， 50 mg/kg goat blood bhi and 5 mg/kg gsbhi， and 5 plates of each media were prepared because isolates of each meida were incubated at 25 ℃， 35 ℃， 36 ℃， 37 ℃， and 38 ℃. growth of sporothrix schenckii was observed and rates of transformation were calculated. 【results】 at 38 ℃， the transformations rates were 25%(the only one strain converted yeast). at 35 ℃ and 37 ℃， the transformations rates were 60% and 85%， respectively. at 36 ℃， the transformations rates were 45%. there were 13 of 20 plates converting yeast when 5% goat blood bhi and 0.5%gsbhi were used， 9 of 20 plates when bhi was used， 4 of 20 plates when sda and pda were used. at 37 ℃， transformation rates of 3 isolates reached 90% on medium of bhi， 50 mg/kg goat blood bhi和5 mg/kg gsbhi; however， the transformation rates of 1 isolate were less than 10% on sda， pda， and bhi. 【conclusion】 temperature is the key factor that affects mycelium-to-yeast transformation of sporothrix schenckii， nourishments of media may also be necessary for transformation. 　　申克孢子丝菌属于双相型真菌，在自然界或室温培养为菌丝相，在体内或3