植物疫苗无致病力青枯雷尔氏菌发酵工艺初步研究.docxVIP

PAGE  PAGE 9 作物青枯病植物疫苗工程菌FJAT-T8发酵工艺的研究基金项目：福建省农科院青年创新基金项目（2010QB-15） 作者简介：陈燕萍（1985-），女，福建省莆田市人，硕士，研究实习员，研究方向为生物技术及生物防治。Tel：0591E-mail：cyp071@ *通讯作者:刘波（1957-），博士, 研究员, 研究方向为微生物生物技术与农业生物药物. Tel：0591E-mail：fzliubo@163.com 收稿日期： 陈燕萍，刘波*，葛慈斌，朱育菁，肖荣凤，史怀 (福建省农业科学院农业生物资源研究所,福州350003) 摘 要：采用30L全自动发酵罐进行植物疫苗工程菌FJAT-T8发酵，并对发酵过程的相关参数进行跟踪检测。以变化显著差异的参数为指标进行聚类，将菌体生长划分为四个周期分别为：0～16h（适应期）、24h（对数期）、32～72h（稳定期）和80～96h（消亡期）；并用多元线性回归及相关法分析各因子间??系，电镜扫描法跟踪菌体形态变化。综合分析，该菌发酵的终止判断指标为：发酵时间第40～48h，发酵液pH值8.7～8.8，溶氧量趋于稳定不变。该研究为提高植物疫苗菌的发酵水平，形成产品质量标准，工厂化生产奠定基础。 关键词：FJAT-T8；状态参数；发酵工艺 Study on fermentation technique of plant vaccine with avirulent strains FJAT-T8 of Ralstonia solanacearum against the bacterial wilt disease CHEN Yan-Ping，LIU Bo*，GE Ci-Bing，ZHU Yu-Jing，XIAO Rong-Feng，SHI Huai (Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China) Abstract: The present paper dealt with fermentation technique of plant vaccine with avirulent strains FJAT-T8 of Ralstonia solanacearum against the bacterial wilt disease. The fermentation process of strain FJAT-T8 was investigated by determination of on-line and off-line parameters in 50L automatic fermentation container. The parameters with the significant differences in the process changes were clustered, the cell growth cycle was divided into four periods etc. 0～16h (adaptation period ), 24h (logarithmic period) ,32～72h (stationary period) and 80 ～96h (decline period). The multiple linear regression and correlation analysis were used to analysis the relationship between fermentation factors. Tracing observation cell morphology was conducted with electron microscope. The comprehensive evaluation indexes for the fermentation were: fermentation time to 40～48h, fermentation pH 8.7～8.8. The dissolved oxygen was decreased essentially from beginning to end of the process. The study was to improve the fermentation of plant vaccine strains, the formation of product quality s