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60%VO2max运动对血液TLR-4及其诱导产物的影响 60VO2 运动对血液 TLR-4 及其诱导产物的影响51015202530354045摘要目的研究 60VO2 运动对血液 TLR-4 mRNA 表达和蛋白水平及其诱导产物的影响通过对 60VO2 运动对免疫功能调节的观察探讨运动与免疫的关系对大众健身等方面的研究提供理论依据方法对 10 名非体育专业大学生进行 60VO2 的跑步机运动在运动前运动后 01224 小时抽取静脉血测定血液中白细胞表面 TLR-4 mRNA 表达和血清 TLR-4蛋白水平血清中 NF-к B促炎因子 IL-6TNF-α 和抗炎因子 IL-4 的蛋白水平结果 1 60VO2 运动后即刻的血液中白细胞表面 TLR-4 mRNA 表达和血清TLR-4 蛋白水平下降血清 NF-к BIL-4TNF-α 蛋白水平上升血清 IL-6 蛋白水平有先升后降的趋势除了 TLR-4 mRNA 表达其他指标都在 24 小时后恢复运动前水平结论 1 60VO2 运动能引起白细胞表面 TLR-4 mRNA 表达和血清 TLR-4 蛋白水平的下降血清 NF-к BTNF-α IL-4 蛋白水平的上升 2 60VO2 运动对有关各免疫指标的影响在运动后 24 小时基本恢复到运动前状态关键词运动人体科学Toll 样受体 4免疫运动中图分类号Q599Effects of 60VO2 exercise on the expression of bloodTLR-4 and its induced productsQIU Tian1 WANG Xintang2 ZHANG Aifang3 1 Sports School Longyan University Longyan Fujian 3640122 The Science Research CenterBeijing Sport UniversityBeijing 1000843 Human movement science SchoolBeijing Sport UniversityBeijing 100084 Abstract Objective Study the effects of 60VO2 exercise on Toll receptor 4 mRNAexpressionthe serum protein level and its induced products influence discuss the relationshipbetween exercise and immunityprovide a theoretical help to public health and some other sportfiledsMethods 10 non-sports majors college students were in 60VO2 exercisetakeblood before exercise and after exercise 01224 hours from venous blood use RT-PCR method totest the blood mononuclear cell surface expression of TLR-4 mRNAuse Elisa method to test theTLR-4 protein level in serumserum NF-кBpro-inflammatory factor IL-6TNF-α andanti-inflammatory factor IL-4 protein level in serumResults 1 Immediately after 60VO2 exerciseblood mononuclear cell surfaceTLR-4 mRNA expression and TLR-4 serum protein levels decreasedserum NF -кBIL-4TNF-αprotein levels riselevels of serum IL-6 protein rise first and then decreasedIn addition toTLR-4mRNA expressionother indicators are return to the levels before exercise within 24 hoursConclusion 1 In this study60VO2 exercise can cause mononuclear cellsurfaces T