实验一 一叶萩碱的提取及鉴定(英文).pptVIP

* Natural Product Chemistry Experiment * Lab Note: Keep Safe and Self-protection Dangerous things: Concentrated acid and basic Organic solvents Fragile glass apparatus Check the apparatus within 15 minutes after the beginning of the experiment Clean up the desk and apparatus after experiment. Sign the name on the Register Form and leave the lab after the checking of teacher. Duty team for cleaning the lab. No Noise * alkaline, alkalinity, basic, basicity hydroin, hydrogen-ion base material sulfonic acid group, sulfuric acid ammonia, ammonium ion elute, elution, milliliter, microliter, shelf percolation, percolate, barrel watch-glass oidin, oidine, potassium iodide, bismuth potassium iodide New words: * 1. Aim Master the principle of Acid- Base extraction method for the alkaloid. Master the principle and operation process for the purification with the ion-extraction resin. Understand the principle and operation process for the percolation and continuous refluxing. Understand the manufacture method for the soft TLC. Experiment One The extraction, purification and identification of Securinine * 2. Requirements Acquire the purified Securinine and identify it with TLC. 3. Principles Alk + H+/H2O → AlkH+ RSO3-H+ + AlkH+ →RSO3-AlkH+ + H+ RSO3-AlkH+ + NH4+OH- →RSO3-NH4+ + Alk+H2O Understand the identification reactions for the alkaloid. Know the isolation and purification methods for the alkaloid with modern and classic technologies. * 4. Introductions Natural Medicine ‘Yiyeqiu’ is the fresh leaves of Securinega suffruticosa (Pall.) Rehd. It mainly distributes in the northeast and central parts of China. It is known to have the function of activate the blood stream. Securinega suffruticosa (Pall.) Rehd. * The alkaloids are the main chemical constituents in Securinega suffruticosa (Pall.) Rehd. and the securinine is one of the alkaloids with highest content. It is used for the treatment of Neuritis (神经炎)， Limb numbness (四肢麻木), Hemiplegia (偏瘫) and Facial Paralysis. (面瘫