抗生素耐药Engineering a virus to select against antibiotic resistanceppt课件.pptVIP

;Drug-Resistant Staph Germs Toll Is Higher Than Thought Wednesday, October 17, 2007; Page A01 ;;Mechanisms of antibiotic resistance;Existing strategies to reduce microbial antibiotic resistance all have significant drawbacks;Project Roadmap;Project Roadmap;Circuit design principles: a stable method for programming phenotype-dependent fitness;Nature has developed ways of detecting low [antibiotic] within cells;Fitness increased ;Bacteriophage lambda can stably alter fitness;Bacteriophage lambda can stably alter fitness;Bacteriophage lambda can stably alter fitness;Bacteriophage lambda can stably alter fitness;A circuit producing [CI] will increase phage immunity and fitness;;PTet;PTet;Our circuit consists of three interconnected pieces;BBa_I744103;tetR;PTet;One full circuit has been constructed that uses feedback regulation;Project roadmap;Using the Gillespie algorithm for modeling the effect of cell type on circuit output;Constitutive promoters produce too much TetR;The model predicts that the the circuit can differentiate cell types;Project Roadmap;tetR;Cellular chassis for characterization;Excitation = 514 nm, Emission = 527 nm;Time (min);Effects of increasing Tc on cell growth;Conclusions;Future Roadmap;COMPUTATION AND APPLIED MATH Steve Cox Jay Raol CHEMICAL AND BIOMOLECULAR ENGINEERING Ken Cox Alec Walker Baylor College of Medicine Bibhash Mukhopadhyay;Linking our cell detection to phage lysis;Ptet Controlled Production of TetR Creates Resistance Phenotype;Tetracycline Affects Growth Rates;Tetracycline Affects on Growth Rates;Phage Circuit Incorporation Relies Upon Recombination Plasmid