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Analysis and Isolation of Adipocytes by Flow CytometryYang LI 2016-04-05Majka SM, Miller HL, Helm KM, et al. Analysis and Isolation of Adipocytes by Flow Cytometry. Methods in enzymology. 2014;537:281-296. doi:10.1016/B978-0-12-411619-1.00015-X.CONTENTSBackgroundAim of the researchDifficulty SolutionBackgroundWhat are adipocytes (fat cells)?Major cells in the adipose tissue.Lipid dropletBackgroundTwo kinds of adipocytes in humanBackgroundProspects of adipocyte biologyLipid store LeptinInhibit eating Increase metabolismBackgroundProspects of adipocyte biologyAdipokinesLipid store Involved in energy metabolismBackgroundProspects of adipocyte biologySpecifically from adipose tissue AdipokinesLipid store Energy metabolismBackgroundProspects of adipocyte biologyAdipokines play an significant role in biological function.BackgroundPrinciple diagramFlow Cytometry (FCM)BackgroundVolume,Morphological complexity of cells, Total DNA/RNA content, Apoptosis, Cell viability, ... ... ... ...Aim of the researchA method for analysis and isolation free-floating adipocytes by Flow Cytometry.Isolation AnalysisFlow cytometry (FCM)Confocal laser scanning microscope (CLSM)Fluorescence-activated cell sorting (FACS)Aim of the researchA method for analysis and isolation free-floating adipocytes by Flow Cytometry.Beckman Coulter--MoFlo? XDP Analysis rate:100,000 events/sec. Sort rate: 70,000 events/sec.AnalysisFlow cytometry (FCM)Confocal laser scanning microscope (CLSM)Aim of the researchA method for analysis and isolation free-floating adipocytes by Flow Cytometry.AnalysisQuantitiveFlow cytometry (FCM)Confocal laser scanning microscope (CLSM)High resolution imaging Aim of the researchA method for analysis and isolation free-floating adipocytes by Flow Cytometry.Imaging Flow Cytometer(Amnis) 12 Images for every cellAnalysisQuantitiveFlow cytometry (FCM)Confocal laser scanning microscope (CLSM)High resolution imaging Aim of the researchA method for analysis and isolation free-floating