芒果苷对自发性高血压大鼠多组织炎症损伤影响-effect of mangiferin on multiple tissue inflammation injury in spontaneously hypertensive rats.docx

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2018-07-27 发布于上海
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芒果苷对自发性高血压大鼠多组织炎症损伤影响-effect of mangiferin on multiple tissue inflammation injury in spontaneously hypertensive rats.docx

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芒果苷对自发性高血压大鼠多组织炎症损伤影响-effect of mangiferin on multiple tissue inflammation injury in spontaneously hypertensive rats

球系膜区无明显扩大，系膜细胞未增多，基底膜厚度尚均匀，未见明显增厚，足突无明显融合。3.与WKY正常对照组比较，心、脑、肾组织模型组TNF-?、iNOS、ICAM-1等mRNA的表达均明显增高（p0.01）；与模型组比较，芒果苷高、中、低及苯那普利组的TNF-?、iNOS、ICAM-1mRNA表达明显降低（p0.01）。结论：芒果苷对于SHR大鼠心、肾的炎症损伤具有保护作用；芒果苷改善SHR大鼠心、肾的炎症损伤，可能与其作用下调TNF-?、iNOS、ICAM-1等mRNA的表达有关。关键词：芒果苷；自发性高血压大鼠；炎症损伤theEffectsofMangiferinonInflammatoryInjuryintheMultipleTissueofSpontaneouslyHypertensiveRatsAbstractObjective:Toinvestigatetheeffectsofmangiferinoninflammatoryinjuryintheheart,brain,kidneyofspontaneouslyhypertensiverat,tolearnmoreaboutthefunctionofmangiferinininjuryinflammatorypreventionandprovideexperimentalbasisforscreeningthedrugofcontrollingthehypertensioninflammatoryreactionseffectively.Methods：Basedonspontaneouslyhypertensiverat,todividethemrandomLyintofourgroups,whichweremodelgroup,highdosemangiferingroup,middledosemangiferingroup,lowdosemangiferingroup,andbenazeprilgroup,thesameageofWKYratasnormalblankcontrolgroup.usinglightmicroscope,transmissionelectronmicroscopetoobservetheratheart,brain,kidneymorphosisofeachgroup;RT-PCRtechniquetodetectthemRNAexpressionofTNF-α、iNOS、ICAM-1intheratheart,brain,kidney.Results：1.ThelightmicroscopyresultsshowedthatthemyocardialfibersofSHRmodelgroupwasmildedema,inflammatorycellinfiltration;glomerulusatrophy,necrosis,lumenswasslightnarrowed;braintissuehadnoobviouschange;highdosemangiferingroup,middledosemangiferingroup,lowdosemangiferingroup,andbenazeprilgroupsignificantlyreducedtheabove-mentionedlesions.TheelectronmicroscopyresultsshowthatthemyocardialcellsofWKYcontrolgroupratwerenormal,mononuclear,oval,chromatindistributedevenly,myofibrilsarrangedorderly,mitochondriaarelargeandround.ThemyocardialcellsofSHRmodelgroupwereapoptotic.Nuclearmembraneofapoptoticmyocardialcellnucleuswasshrinkage,chromatincondensation,margination,aggregationatthenuclearmembranewaspatchy;partofthenucleolusdisappeared;occasionalnucleuscondensation.Themyofibrilisvague,disorganized,andpartiallydissolvedonbothsidesoftheintercalateddisc,partofthecardiacmusclecellmitochondrialswelling,cristaedisorde