菩人丹超微粉对糖尿病大鼠视网膜ang-2 tnf-α表达的影响-effect of pu ren dan ultrafine powder on expression of ang - 2 tnf - α in retina of diabetic rats.docxVIP

中文摘要佳，神经节细胞排列紊乱，细胞数量减少，可见细胞空泡样变、核固缩等现象。PRD治疗组视网膜内界膜表面粗糙欠平，轻度肿胀增厚、视网膜分层较清晰，神经节细胞排列轻度紊乱，细胞数量轻度减少。3.各组大鼠视网膜Ang-2的表达3.1SP免疫组织化学染色结果：Ang-2蛋白免疫阳性产物呈棕黄色颗粒状，表达位于细胞浆。Ang-2表达阳性细胞主要分布在大鼠视网膜神经节细胞层及内核层。与正常对照组相比糖尿病模型组胞浆Ang-2免疫阳性产物呈深棕黄色；PRD治疗组Ang-2免疫阳性产物表达较糖尿病模型组明显减弱，胞浆呈棕黄色。3.2采用MiVnt图像分析系统对免疫组化阳性表达面积进行定量分析结果：与正常对照组相比糖尿病模型组阳性表达面积明显增加（P＜0.01）；PRD治疗组与糖尿病模型组相比，阳性表达面积减少（P＜0.01）。3.3Westernblot法检测Ang-2蛋白表达结果：与正常对照组比较，糖尿病模型组大鼠视网膜Ang-2蛋白表达明显升高（P＜0.01）；PRD治疗组大鼠视网膜Ang-2蛋白的表达与糖尿病模型组比较明显降低（P＜0.01）。4.各组大鼠视网膜TNF-α的表达4.1Westernblot法检测TNF-α蛋白表达结果：与正常对照组比较，糖尿病模型组大鼠视网膜TNF-α蛋白的表达明显升高（P＜0.01）；PRD治疗组大鼠视网膜TNF-α蛋白的表达与糖尿病模型组比较明显降低（P＜0.01）。4.2RT-PCR检测TNF-αmRNA表达结果：与正常对照组比较，糖尿病模型组大鼠视网膜TNF-αmRNA的表达明显升高（P＜0.01）；PRD治疗组大鼠视网膜TNF-αmRNA的表达与糖尿病模型组比较明显降低（P＜0.01）。结论：PRD可以改善糖尿病大鼠视网膜病变，这一作用可能是通过下调糖尿病大鼠视网膜Ang-2和TNF-α的表达，从而减少视网膜微血管渗漏及病理性新生血管生成，发挥其对糖尿病大鼠视网膜微血管损伤的保护作用。关键词：菩人丹超微粉；糖尿病视网膜病变；血管生成素-2；肿瘤坏死因子-α；大鼠EffectsofPurendansuperfinepowderontheexpressionofAng-2andTNF-αinretinalofdiabetesratsABSTRACTPURPOSEThroughinginvestigatingtheeffectofpurendansuperfinepowderontheexpressionofangiopoietin-2(Ang-2)andtumornecrosisfactor-α(TNF-α)inretinalwithdiabetesrats,todiscusstheprotecteffectiveofPRDonthediabetesrats’retinalcapillarypathologicalchanges.METHODSThirtysixmaleWistarratsweredividedinto3groupsrandomly:normalcontrolgroup,diabeticmodelgroupandthePRDtreatmentgroup,eachgroupincludes12rats.Usetheintraperitonealinjectionof2%Streptozotocin(25mg/kg·d)onthePRDtreatmentgroupanddiabeticsmodelratsfor3dayscontinually,toestablishtypeⅡdiabetesratsmodel.Thebloodglucose≥16.7mmol/Lwastakenasastandard.Afterestablishingthemodelsuccessfully,thediabetesmodelgroupwasintragastricadministrationwithsolventswithoutPRD;andtheratsinPRDtreatmentgroupwereintragastricadministrationwithPRD(1.8g/kg/d)for12weeks.Theratsinnormalcontrolgroupwerebredasnormal.ONETOUCHbloodglucosemeterwasusedtodetectthebloodglucoseofratsineachgroup;thehematoxylin-eosin(HE)stainingwasusedtoobservehistopathologicalchangeinretinaofratsineachgroup;theSPimmunohistochemicalstainingandWe