前列腺癌中glypican-3 β-catenin和gsk-3β的表达及 意义-expression and significance of gly pican - 3beta - catenin and gsk - 3beta in prostate cancer.docxVIP

目录中文摘要········································································································1英文摘要········································································································4研究论文前列腺癌中Glypican-3、β-catenin和GSK-3β的表达及意义前言········································································································9材料与方法···························································································10结果·······································································································15附图·······································································································17附表·······································································································25讨论·······································································································28结论·······································································································35参考文献·······························································································36综述经典Wnt信号通路与恶性肿瘤的关系··········································45致谢···············································································································63个人简历·······································································································64前列腺癌中Glypican-3、β-catenin和GSK-3β的表达及意义摘要目的：前列腺癌(Prostatecancer，PCa)是老年男性常见的恶性肿瘤之一，在欧美发达国家前列腺癌的发病率居男性肿瘤的第一位，其导致的死亡率仅次于肺癌。我国前列腺癌发病率虽低于西方国家，但随着我国社会人口老龄化的加速，居民饮食结构的改变及前列腺癌筛查手段的普及，发病率与癌症检出率呈逐年升高趋势，而且我国大多数地方新确诊前列腺癌中晚期患者多于欧美国家，这对我国前列腺癌患者治疗效果及预后产生了重要影响[1-3]。因此，进一步探索前列腺癌的发病机制，探寻新的早期诊断及治疗方法具有重要意义。研究发现磷脂酰肌醇蛋白聚糖-3(Glypican-3，GPC-3)在前列腺癌组织中呈高表达，而且与经典Wnt信号通路关系密切，有望成为前列腺癌新的肿瘤标志物。本研究通过检测磷脂酰肌醇蛋白聚糖-3(Glypican-3，GPC-3)、β-链蛋白(β-catenin)和糖原合成酶激酶-3β(GSK-3β)蛋白在前列腺癌及良性前列腺增生组织中的表达情况，分析GPC-3、β-catenin和GSK-3β蛋白在前列腺癌中的意义及其与经典Wnt信号通路可能的关系，为前列腺癌探索新的肿瘤标志物；通过检测前列腺癌患者血清GPC-3蛋白的表达水平，探讨GPC-3联合PSA检测在前列腺癌患者中的临床价值，为提高前列腺癌临床诊断的准确性探索新方法。方法：采用