妊娠期dehp暴露对小鼠胎盘生长与发育影响-effect of dehp exposure during pregnancy on placental growth and development in mice.docxVIP

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妊娠期dehp暴露对小鼠胎盘生长与发育影响-effect of dehp exposure during pregnancy on placental growth and development in mice.docx

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妊娠期dehp暴露对小鼠胎盘生长与发育影响-effect of dehp exposure during pregnancy on placental growth and development in mice

Ab Abstrct PAGE PAGE IV ABSTRACT Objective: Di-(2-ethylhexyl) phthalate (DEHP) is used as a plasticizer and widely dispersed in the environment. DEHP is non-covalently bound to plastics, and therefore, it will leach out of these products after repeated use, heating, and/or cleaning of the products, allowing the toxicant to be ingested through inhalation, ingestion and dermal contact on a daily basis, and harming to animal and human reproductive system. Studies have shown that maternal exposure to DEHP reduces endometrial receptivity and embryo implantations, increases resorptions, and decreases fetal weights of offspring. However, no detailed information is available about the effect of DEHP on the placentation during the pregnancy. Therefore, this work was designed to evaluate the impact of DEHP on the placenta and embryo development by gavage in vivo. Methods: Mice were administered DEHP by gavages at 125, 250, 500 mg/kg/day from gestational days (GD) 1. Treated mice were sacrificed for uterus, embryo and placenta collection at GD 9 and 13. Hematoxylin and eosin staining was observed the effect of DEHP on the histopathology of placenta. Immunohistochemistry was used to examine the effect of DEHP on the proliferation of placenta and labyrinth vascularization; The expressions of marker genes in placenta were analyzed by real-time quantitative PCR; Western blot was to investigate the effects of DEHP the apoptosis of placenta and related signaling pathways. Results: At GD 9, treatment with DEHP did not significantly affect the weight of embryo enclosed by deciduae and the ratio of embryo vs. body weight compared to control group (P0.05). However, at GD 13, DEHP treatment significantly reduced the weight of embryo plus placenta and the ratio of embryo plus placental weight to body weight (P0.05 or 0.01),and placental weight and the ratio of placental weight to body weight compared to control group (P0.01). PAGE PAGE V At GD 9, DEHP treatment signi