肿瘤转移新进展ppt课件.ppt

A and B, 2D-DIGE analysis was carried out using 50 mg of total protein from MB-231-Pa（ parental MDA-MB-231 cell line ） and MB-231-Br cells labeled with Cy3 (green) and Cy5 (red), respectively, and resolved on the same gel. Pulled sample containing equal amounts total cellular proteins from both cell lines labeled with Cy2 was used as an internal standard (image not shown). C, Number of spots consistently exhibiting differential expression between MB-231-Pa and MB-231-Br cells in 4 independent experiments depending on DeCyder 7.0 settings (average fold difference). 2D-DIGE analysis of MB-231