水溶性量子点cdse与cdsezns的合成及其分析应用-synthesis and analytical application of water-soluble quantum dots cdse and cdsezns.docxVIP

AbstractQuantumdots(QDs)orsemiconductornanocrystalsarespecifickindofnanoparticleswhichhavebeendevelopedinrecentyears．Theyareidealbioconjugatedfluorescencedyebecauseoftheirspecialopticalfeaturessuchasbroaderexcitationspectrum,narroweremissionspectrumandlongerlifetime．SynthesisandapplicationofQDshavegeneratedatremendousamountofinterestsinthefieldsofnanoanalyticalchemistryandbioanalyticalchemistry．Chapter1isthereviewaboutsynthesisandapplicationsofQDs，andchapter2-6areresearchworksoftheauthorduringthestudyofmaster’sdegree.CdSeQDsweresynthesizedinaqueoussolutionusingCdCl2,NaHSeasprecursorandmercaptoaceticacid(MAA)asstabilizer.Theeffectofreactiontime,reactiontemperatureandthemolarratioofCd:Sewerealsostudied．ThesynthesizedQDswerecharacterizedbyultraviolet-visible(UV-vis)absorptionspectra,fluorescence(FL)spectraandtransmissionelectronmicroscope(TEM).TheresultsshowedthatCdSeQDsweremono-dispersedwithaveragesizeabout2nm.GoodFLsignalofCdSeQDscanbeobservedwhentheratioofCd:Seat2,thetemperatureat30℃andthereactiontimeat40min．BasedonthecharacteristicfluorescencequenchingoftheCdSeQDsbyVitaminB1，asimple,rapid,sensitiveandselectivequantificationmethodwasproposedfordeterminationofvitaminB1forthefirsttime.Underoptimumconditions,alinearrelationshipwasobservedbetween0.5-60.0μg/mL,withacorrelationcoefficientof0.9941andthelimitofdetectionwas15ng/mL．TheproposedmethodwasappliedtodeterminationvitaminB1inmedicamentsamplesandobtainedsuccessfulresults．TheCdSe/ZnScompositequantumdots(QDs)thatcappedwithL-cysteineweresynthesizedandappliedforultrasensitivePb2+sensing.Thefluorescenceoftheas-obtainedCdSe/ZnSQDswasquenchedonlybyPb2+.Underoptimalconditions,theresponseoftheopticalsensorwaslinearlyproportionaltotheconcentrationofPb2+rangingfrom1.60μg/Lto240.0μg/L.Thedetectionlimitwas0.23μg/L.Arelativestandarddeviationof0.13%for7replicatesofa20μg/LPb2+solutionwasobtained.TheproposedmethodhasbeensuccessfullyappliedtothedetectionofPb2+inwatersamples.Keyword:Quantumdots;Synthesis;VitaminB1;Leadion目录第1章绪论........