水稻“紫色互补”性状的遗传研究及其在纯度检测中应用-genetic study on.docxVIP

万方数据 万方数据 Abstract Accurate, rapid and economical method of purity identification hybrids is one of the important measures to protect the safety of hybrid rice production. The period of field identification in Hainan is too long and limit sale. And the results of two identification methods through observing grain shape and phenol staining are not accurate. The protein electrophoresis method also failed to be large-scale used because of poor stability and less polymorphic. Tedious process，expensive equipment，high-quality personnel limit the DNA marker method to be used widely. Another way to identify seed purity is molecular design breeding. Generally the design makes HYPERLINK /dict_result.aspx?searchword=%e4%b8%8d%e8%82%b2%e7%b3%bbamp;tjType=sentenceamp;styleamp;t=male%2Bsterile%2Bline sterile line carry a recessive trait and father carrying dominant trait. This design can effectively distinguish the seeds from sterile line selfing with hybrid F1 seeds. But the design failed to distinguish the seeds coming from mixing and other pollens, and easily lead to high false-positive test results. During we screened heterosis between Indica rice and Japonica rice, purple complementary phenomenon was been observed. The genetic mechanisms leading to the phenomenon was analyzed. And applications of the phenomenon in purity identification of hybrid rice were demonstrated by examples. The main conclusions were as follows: Japonica rice GH102 and many Indica rice had non-purple coleoptile, apiculus and stigma. When GH102 crossed with these Indica rice, the F1 had purple lines on coleoptile. F2 seeds showed purple apiculus and stigma. The apiculus and stigma of F1 seeds were not purple because they developped from sterile line and had the same genotype with sterile line. We called the phenomenon as Purple complementary that both parents had no purple characters but their HYPERLINK /dict_result.aspx?searchword=%e5%90%8e%e4%bb%a3amp;tjType=sentenceamp;styleamp;t=progenies progenie