水稻phd-finger家族低温诱导基因的鉴定与启动子功能分析-identification and promoter function analysis of low temperature inducible genes in paddy rice phd - finger family.docxVIP

万方数据 万方数据 Abstract Rice is one of the most important crops in the world. The high and stable yield of the rice is related to national security. Although the rice yield and quality are always destroyed by abiotic stresses, such as cold, drought and salinity. The breeding process of oriented cultivation and directed modification will be shortened via modern genetic technology engineering. It is one of the most effective approaches to improve the resistance-capability by using explored candidate functional genes. Transcription factors in plants play a very important role in stress response to adversity stress. It becomes a hotspot to research on TFs and their function in the genetic improvement of plant resistance breeding. PHD-finger transcription factors family is a kind of zinc finger protein widely exists in eukaryotic. The researches of their function often focus on gene transcription and chromatin state regulation. Thus, there were fewer reports about their function involved in stress response. So it’s significant for adversity-resistance breeding in rice by comprehensively identifying the members of PHD-finger in rice, exploring the candidate genes in reponse to abiotic stress and explaining the resistance mechanism. In this study, we performed genome-wide survey of PHD-finger gene members in rice and characterized their gene structure features, phylogenetic relationships and abiotic stress-induced expression patterns. Two genes in response to coldi-induced were screened, and then the function and acting mechanism of their promoters were also analysed. The detailed results were as follows: By Blast homologous search, a total of 58 PHD-finger transcription factors were identified in the rice genome. They were found to distributed unevenly on chromosome 1 to 12 except chromosome 10 by chromosomal location. About 1/4 of the family member genes were distributed near the telomeric region. Additionally, there were 6 gene clusters with contribute to the expansion of PH