设计mega press序列在体检测脑内γ 氨基丁酸的研究-study on design of mega press sequence for in vivo detection of γ -aminobutyric acid in brain.docxVIP

摘要磁共振频谱（Magneticresonancespectroscopy,MRS）技术作为一种无损非介入在体检测脑内代谢物的方法已有相当一段历史。由于人脑中γ-氨基丁酸(γ-aminobutyricacid,GABA)的浓度较低及其他代谢物信号的干扰，基于MRS技术在体定量GABA尚未报道出成熟有效的方法。目前，这一研究课题在3T、4T、7T等超高场分别有不同程度的研究结果。然而，在临床1.5T磁共振扫描仪上，GABA的在体定量检测却鲜为少见。本研究根据代谢物GABA的特性，基于乘积算符理论和相干路径选择理论，研究设计出MEGA-PRESS（Mescher-Garwoodpoint-resolvedechospinsequence）序列。在临床1.5T磁共振扫描仪上进行了模型和活体实验，验证了MEGA-PRESS溶剂抑制技术的滤波机制及设计出的序列在体定量人脑代谢物GABA的可行性。结果证实基于GE公司EPIC软件能提取被其它高信号淹没的低浓度成分的代谢物，可从肌酸背景中分离出3.0ppm处GABA。针对我国目前大多数医院仅拥有1.5T磁共振的状况，这一研究结果为临床开拓定量检测大脑GABA等代谢物的浓度提供了一条新途径。同时，为探索代谢物GABA引起相关疾病的新治疗方法提供了理论依据。关键词：磁共振频谱；γ-氨基丁酸；MEGA-PRESS序列；脉冲序列设计；AbstractAsanon-destructiveandnon-invasiveanalyticaltechnique,MagneticResonanceSpectroscopy(MRS)technologyhasbeentheoptimumselectionfordetectingbrainmetabolitesfordecades.However,theconcentrationofthismetaboliteγ-aminobutyricacid(GABA)inhumanbrainisquitelow.What’sworse,GABA’sweaksignalssufferfromlargeoverlapofothermetabolitesignals.However,thereisnotamatureandeffectivemethod,whichisinvivomeasuringtheconcentrationofGABAbasedonMRS.Atpresent,therearesomeresearchesonthisprojectinhigherfieldmagneticresonancescanners,suchas3T,4T,7TMRIscanners.But,on1.5TMRIscanners,thereisnotanyexactlyreportinthisfield.Inthisstudy,MEGA-PRESSsequenceisdesignedtodetectGABAinvivoonaclinical1.5TMRIscanner.ThesequenceisaccordingtothecharacteristicsofGABAandthecoherentpathchoiceofproductoperatortheory.Then,modelandinvivoexperimentswerecarriedoutwiththeMEGA-PRESSsequenceon1.5Tscanner.TheexperimentresultsverifiedthatthefilteringmechanismoftheMEGA-PRESSsolventsuppressiontechniqueisfeasibleofhumanmetabolismGABA’squantitationinvivo.Also,itcanbeconfirmedthatthetechnique,basedontheGEcompanyEPICsoftware,couldexactlybringoutlowconcentrationofmetabolitefromthestrongsignal,suchasseparatedGABAfromthebackgroundofCrat3.0ppm.ConsideringthesituationinChina,mosthospitalsjustowna1.5TMRIscanner,MEGA-PRESSsequenceprovidesanewmethodforthequantitationofhumanbrainsGABA.Furthermore,theresearchcouldprovideareliabletheoreticalbaseforthenewtrea