湿困脾胃证动物模型规范化的实验分析-experimental analysis on standardization of animal model of damp-trapped spleen and stomach syndrome.docxVIP

中文摘要实验目的检验湿困脾胃证动物模型的可重复性及稳定性，使模型实现规范化；对模型作进一步实验指标检测，更深入地阐释湿困脾胃证的本质。实验方法按照增加湿度、饮食调控结合睡眠控制的多因素造模方式复制出湿困脾胃证动物模型并进行药物反证，观测宏观体征，对物质能量代谢、水电解质代谢等客观指标进行检测和结果统计分析。实验结果大鼠在造模后出现了类似湿困脾胃证的宏观症状体征；模型血清Na、Cl含量降低，糖、蛋白、脂质代谢紊乱，肾素－血管紧张素－醛固酮系统（RAAS）分泌增加。经平胃散治疗后模型病变明显恢复，效果好于自然恢复组。实验结论成功复制出大鼠湿困脾胃证候模型，证明了造模方法的可重复性和稳定性，新指标的检测反映了湿困脾胃证的部分实质。关键词湿困脾胃证；动物模型；肾素活性（PRA）；血管紧张素(Ang)；醛固酮(ALD)；心钠素(ANF)ABSTRACTObjective：Authenticatethedampnessobstructingspleen-stomachpatternanimalmodelsrepeatabilityandstability,tomakeitstandardized;testthemodelanimalsmorelaboratoryindexsign,hopetoexplainfurthertheessenceofthepattern.Methods：Inaccordancewiththeincreaseinhumidity,dietandsleepcontrolofthemulti-factormodeltoduplicatethedampnessobstructingspleen-stomachpatternmodelsandapproachtodrugevidence,observingsignsofmacroscopicphysical,objectiveindicatorssuchasthematerialandenergymetabolism,thewaterelectrolytemetabolismfortestingandstatisticalanalysisofresults.Result：Ratsappearasimilardampnessobstructingspleen-stomachpatternssymptomsandsignsofmacroscopicinthemodelsafter;andmodelratsserumNaandClcontentlower,themetabolismofsugar,proteinandfataredisorderly,theRAASexcretesincrement.AftertreatbythePing-Wei-San,modelsdiseaseobviouslyresumebetterthanthenaturalrecoverygroup.Conclusions：Thedampnessobstructingspleen-stomachpatternmodelswereduplicatedsuccessful,therepeatabilityandstabilityofthemethodofmodelingcanbeproved,thenewlaboratorysignsasRAAScanreflectpartoftheessenceofthedampnessobstructingspleen-stomachpattern.Keywords：Thedampnessobstructingspleen-stomachpattern;Animalmodel;Reninactivity;Angiotension;Aldosterone;AtrialNatriureticFactor缩略词表缩略语英文全称中文全称RAASRenin－Angiotension－Aldosteronesystem肾素－血管紧张素－醛固酮系统IgAImmunityglobulin-A免疫球蛋白-AANFAtrialNatriureticFactor心钠素ReninRenin肾素PRAPlasmaReninActivity肾素活性ALDAldosterone醛固酮NTNeurotensin神经降压素ADHAntidiureticHormone抗利尿激素SILSolubleIL-2receptor可溶性IL-2受体IL－6Interleukin-6白细胞介素HPHelicobacterpylori幽门螺杆菌GluGlucose葡萄糖TPTotalProtein总蛋白TGTriglycerides甘油三脂CHOCholesterol总胆固醇HDLHDL-Cholesterol高密度脂