红毛七的抗炎、镇痛作用研究.docVIP

红毛七的抗炎、镇痛作用研究 　　[摘要] 目的 考察红毛七的抗炎、镇痛作用。 方法 将小鼠按?w重随机分为模型对照组（CMC-Na），阳性对照组，红毛七水提取物低、中、高剂量组（3、9、27 g/kg，以生药计），每组各10只，灌胃给药，每天1次，连续7 d。采用二甲苯致小鼠耳肿胀法测定小鼠耳肿胀度；采用纸片致肉芽增生模型，测定小鼠肉芽肿抑制率，以考察红毛七水提取物的抗炎作用；采用乙酸扭体法测定小鼠扭体反应潜伏期及15 min内扭体次数；采用热板法测定小鼠给药前及给药30、60、90、120 min后痛阈值，以考察红毛七水提取物的镇痛作用。 结果 与模型对照组比较，红毛七水提取物中、高剂量组可减轻小鼠耳肿胀度（P 0.05或P 0.01），高剂量组可以减轻肉芽组织干重（P 0.05），红毛七水提取物中、高剂量组可延长小鼠扭体反应潜伏期并减少扭体次数（P 0.05或P 0.01），提高给药30、60 min后的痛阈值（P 0.05或P 0.01），且高剂量组可以提高给药90、120 min后的痛阈值（P 0.05或P 0.01）。 结论 红毛七水提取物有一定的抗炎、镇痛作用。 　　[关键词] 红毛七；水提取物；抗炎；镇痛；动物药效学 　　[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210（2017）09（a）-0026-04 　　[Abstract] Objective To investigate the anti-inflammatory and analgesic effects of Caulopyhllum robustum water extract. Methods The mice were randomly by weight divided into model group （CMC-Na）， positive control group， Caulopyhllum robustum water extract low-dose， medium-dose and high-dose groups （3， 9， 27 g/kg， calculated by crude drug）， each group had 10 mice. They were given relevant medicine intragastrically， once a day， for consecutive 7 d. The xylene-induced ear edema method was used to determine the degree of ear edema. Paper-induced granuloma model was used to determine the inhibition ratio of granulation tissue hyperplasia， the anti-inflammatory activity of Caulopyhllum robustum water extract was investigated. The pain incubation period and the number of writhing within 15 minutes were determined by acetic acid writhing method. Pain thresholds of mice were determined by hot-plate test before and after medication 30， 60， 90， 120 minutes， the analgesic effect of Caulopyhllum robustum water extract was investigated. Results Compared with model group， Caulopyhllum robustum water extract medium-dose and high-dose groups could restrain the degree of ear edema of mice （P 0.05 or P 0.01）； the high-dose group could decrease the dry weight of granulation tissue （P 0.05）. Caulopyhllum robustum water extract medium-dose and high-dose groups could prolong the pain incubation