江西建昌帮姜天麻与其他炮制品中天麻素的含量差异.docVIP

江西建昌帮姜天麻与其他炮制品中天麻素的含量差异 　　[摘要] 目的 建立天麻炮制品中天麻素含量的测定方法，比较姜天麻与其他炮制品中天麻素的含量差异。 方法 采用高效液相色谱法，色谱柱Ultimate3000 C18柱（4.6 mm×250 mm，5 μm），流动相乙腈-水（3∶97），检测波长220 nm，流速1 mL/min，柱温25℃。 结果 天麻素的进样量和峰面积呈现良好的线性关系，平均回收率为99.76%，RSD为1.422%，姜天麻、生天麻、硫磺熏蒸天麻中天麻素的含量分别为1.001%、0.458%、0.406%。 结论 姜天麻与其他不同炮制品中天麻素含量差异较大。本法灵敏，快速简便，可为深入研究天麻提供参考。 　　[关键词] 建昌帮；天麻；天麻素；生姜；含量 　　[中图分类号] R917 [文献标识码] A [文章编号] 1673-7210（2018）02（a）-0027-04 　　The content difference of gastrodin between Gastrodia Rhizoma prepared by ginger from Jianchang Faction and other processed products in Jiangxi Province 　　LU Ping1 JIN Lei1 JIA Caihong1 LI Xuran2 NIAN Fenghong2 GONG Qianfeng2 YE Xide2 　　1.Department of Pharmacy， the 105th Hospital of Chinese People′s Liberation Army， Anhui Province， Hefei 230031， China； 2.College of Pharmacy， Jiangxi University of Traditional Chinese Medicine， Jiangxi Province， Nanchang 330004， China 　　[Abstract] Objective To establish a method for detecting the contents of gastrodin in processed products of Gastrodia Rhizoma， and to compare the content difference of gastrodin between Gastrodia Rhizoma prepared by ginger and other processed products. Methods High performance liquid chromatography method was conducted for the analysis. The chromatographic column was Ultimate3000 C18 （4.6 mm×250 mm， 5 μm）， the flowing phase was acetonitrile-water （3∶97）， the detection wavelength was 220 nm， the flow rate was 1 mL/min， and the column temperature was 25℃. Results The sample amount and peak area of gastrodin showed good linear relationship， the average recovery rate was 99.76%， RSD was 1.42%， the contents of gastrodin in Gastrodia Rhizoma prepared by ginger， raw Gastrodia Rhizoma and Gastrodia Rhizoma prepared by sulfur fumigation were 1.001%， 0.458%， 0.406% respectively. Conclusion The contents of gastrodin in Gastrodia Rhizoma prepared by ginger shows significant differences with other processed products. This method is sensitive， fast and easy， which can provide reference for further research of Ga