双向电泳D技术相关.pptVIP

2D Electrophoresis:1st Dimension;Proteomics Experimental Workflow;Isoelectric focusing - IPG rehydration;First dimension – what is the isoelectric point (pI)?;Proteins move in a pH gradient until they reach their isoelectric point (pI);How do you run IEF?;IPG Strips;IPG strips;Broad Range pI Separations;IPG Strip Selection;IPG Strip Resolution;Sample/Rehydration buffer for IEF;Laemmli Sample Buffer for 1D SDS;Protein Solubility ;Disruption of Disulfide Bridges;Disruption of Ionic Bond Hydrogen Bond;Disruption of Hydrophobic Interactions;Sample/Rehydration buffer for IEF;Isoelectric focusing – rehydration sample loading;Pros and cons of different rehydration techniques;Power conditions/programming;Questions?