铝致tau蛋白异常磷酸化作用及机制分析-analysis of abnormal phosphorylation of tau protein induced by aluminum and its mechanism.docx

??tau?????????????おЁ???ǐ??ǐ???1?????????????????MCI??????????????????tau??????????MCIП??????2??????偠????????哴?д?????????????tau???????????????3?????お????偠??????????偠????⑤???????????SH-SY5Y????????▔?-??CaMK-?????KN93??????ˉ2A?PP-2A?▔???????????tau?????????お??tau??????????????1?????????????66??????????????????????????????70??????有??????MMSE???MCI??????????お??ぎ?????????????????ㄝ??????ICP-MAS??????????????????????westernblot??????P-tau181?231?262?396????????tau?tau5???∈????????????MCI????P-tau181?231?262?396?tau5?????????????????????MMSE??????????∈??MCI????????MMSE?????∈??P-tau181?231?262?396?tau5????????2?????ч??????哴?320?????????4????80????????ㄐ但???AlCl3??但?Ё???哴???催?Ё???????????120?12?1.2mg/kg.d??????但???????2?????????????????∈???偠????偠????偠???哴??????????????????哴???????????ㄝ??????ICP-MAS??????∈?????????HE????????哴???????????????Western-blot???哴???P-tau181?231?262?396?tau5?????????????????哴?д??????????????????P-tau181?231?262?396?tau5??????????3???SH-SY5Y????????偠????ぎ????200?0$O3+?400?0$O3+?800?0$O3+?800?0$O3++0.5?0.N93?800?0$O3++5nM??????800?0$O3++DMSO?DMSO?????乘48??????乍?????CCK-8??????????Flu-3AM???????????????Ca2+????ELISA????CaM???CaMK-????PP-2A??????????Western-blot??????P-tau181?231?262?396?tau5???????CaMK-????KN93?PP-2A▔?????????Tau??????????????????1?????????????25.18±2.65μg/L??????????9.97±2.83μg/L??t?偠?????????∈???催?????P0.01???2?MMSE??????????????????26.13±2.57?????27.89±1.91???????????Н?P0.05??MMSE???????????????????????????????????????Ё???????????????????????????????P0.05????????????????3?????MCI????18.2%?????MCI????5.7%???MCI??????????Н?P0.01???????催??????4?MMSE????????????????啘????????∈??P0.01???5?logistic??????????お??MCI???????????OR=1.168?OR?95%CI?1.136~1.178?P0.01???6?Western-blot????MCI???tau5?P-tau181?P-tau231?P-tau396???????催??MCI????????P-tau181?P-tau231?P-tau396??????催?????P-tau262????????????Н?????偠??1?∈??┰???????????????∈??┰?????????Н?P0.01??????????????哴∈??┰??????????Ё?催???∈???偠┰????催????(P0.05)???????∈??┰?????????Н?∈??こ??????????????????哴