微流控芯片水力门控及其生物分析分析-hydraulic gating of microfluidic chips and its biological analysis.docx

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2018-08-07 发布于上海
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微流控芯片水力门控及其生物分析分析-hydraulic gating of microfluidic chips and its biological analysis.docx

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微流控芯片水力门控及其生物分析分析-hydraulic gating of microfluidic chips and its biological analysis

proposedanimprovedcellsorterusingtheadjustable-interfacemode,whichhaspotentialtoimprovepurity,throughputandcellviability.Inthecellstimulation,weestablishedachemicalsignalgeneratorbythepinchedmodeforchemicalstimulationofasinglecelllocatedintheanalysischannel.Systemevaluationrevealedthattherisetimeandthefalltimeofthesolutionexchangewere19.8±0.8msand20±1msrespectively.Asaproofofconcept,adenosine-triphosphatestimulationofHeLacellswasdemonstratedbyinvestigatingP2Y-IP3signalingpathwaymediatedcalciumrelease.Furthermore,preliminarystudieshavebeenmadeonthefull-channelmodeandtheadjustable-interfacemodeforcellstimulation.Thefull-channelmodehaspotentialforchemicalstimulationofmultipleindividualcellsintheanalysischannelsimultaneously.Theadjustable-interfacemodeenablescellstimulationinaspatio-temporallycontrollablemannerwithatemporalresolutionoflessthan43msandaspatialresolutionof3.4±0.2μm,whichcanbeusedtoinvestigatesub-cellularcelldynamicsorcellcommunication.Inthedropletpurification,weintegrateddropletgenerationandsortingonasinglechipforafluorescence-activateddropletsorter.Dropletgenerationwasachievedwithathroughputof18droplets/s;dropletsortingwasrealizedbytheadjustable-interfacemodewithbothpurityandrecoveryrateofmorethan99%.Moreover,weproposedhydrodynamicgatingbasedonanaqueous-oilmicrofluidicsystemforselectiveencapsulationmicrobeadsintopicoliter-scaledroplets.Theresultindicatedapurityof92%andarecoveryrateof96%.Atentativeresearchonopen-tubularliquidchromatographicseparationwasalsoconductedbythepinchedmode,andthreekindsoffluorescentdyeswereresolvedusingnativepoly(dimethylsiloxane)asasoildphaseandacetonitrileasamobilephase.Insummary,hydrodynamicgatingwerevalidatedasaversatileanduniversalstrategyformicrofluidicsampleintroduction,anditwasexpectedtobeapowerfulplatformtechnologyforbioanalyticalapplicationsoflab-on-a-chip.Keywords:Lab-on-a-chipMicrofluidicchipHydrodynamicgatingSampleintroductionCellanalysisBioanalysis独创性声明本人声明所呈交的学位论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除文中已经标明引用的内容外，本论文不包含任何