微生物在生物栅处理景观水体中的作用及机理分析-function and mechanism analysis of microorganism in biological barrier treatment of landscape water.docxVIP

ABSTRACT As the high-speed development of economy and society, most scenic water is seriously polluted and the capability of self-purification is decreased in recent years. Therefore, it is particularly important to solving the eutrophication and restoring the ecological function of landscape water. The ecological restoration technology of scenic water has become a hot topic at domestic research. In order to study the purification effects, a pilot biological grid device was built and different plant biological grid system were set to compare the difference of purification effects on eutrophic landscape water. In order to study the role and mechanism of microorganism on contaminant removal in biological grid systems, four small pilot devices, which were respectively filling group, Irispseudoacorus floating-beds, Irispseudoacorus biological grid, Cannaindica biological grid, were constructed during the dynamic experiments. The dynamic characteristics of each small pilots treatment efficiency on landscape water, the number of bacteria and fungi, and the activity of dehydrogenase were continuously monitored. At the beginning and end stage of the four pilot devices the roots of plant and fibrous fillers were selected. The molecular biological method of PCR-DGGE was applied to analyze the microbial community structure and diversity of those samples and some bands of the dominant species were recycled and sequenced. The sequencing results were imported into the BLAST and the phylogenetic tree was constructed. Comparing the purification effects of different plant biological grid system on landscape water, the results indicate that Cannaindica biological grid has a higher removal rate. The dynamic operation conditions of the four small pilot devices are that HRT is 4 days and influent flow is 8.6 L/d, which continue more than 3months. The results show that the purification effects become better and more stable with the increasing of running time. The average removal efficien