苦丁茶叶化学成分研究.PDFVIP

• 18 • 中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 1 期 2011 年 1 月 苦丁茶叶的化学成分研究 1, 2* 1 1 1 2 1* 左文健 ，陈惠琴 ，李晓东 ，王振辉 ，戴好富 ，王金辉 1. 沈阳药科大学中药学院，辽宁 沈阳 110016 2. 中国热带农业科学院热带生物技术研究所 农业部热带作物生物技术重点开放实验室，海南 海口 571101 摘 要：目的 研究苦丁茶 Ilex kudingcha 叶的化学成分。方法 利用硅胶柱色谱、大孔树脂柱色谱、Sephadex LH-20 凝胶 色谱、制备 HPLC 进行分离，通过波谱数据和理化性质进行化合物结构鉴定。结果 共分离得到 8 个化合物，分别鉴定为 mateglycoside D （1）、咖啡酸甲酯（2 ）、香草醛（3 ）、没食子酸（4 ）、咖啡酸（5 ）、β-谷甾醇（6 ）、对羟基苯甲醛（7 ）、阿 魏酸（8 ）。结论 化合物 2 、7 为首次从冬青属植物中分离得到，化合物 1、3 、4 、8 为首次从该植物中分离得到。 关键词：苦丁茶；冬青属；mateglycoside D ；香草醛；没食子酸 中图分类号：R284.1 文献标志码：A 文章编号：0253 - 2670(2011)01 - 0018 - 03 Chemical constituents of Ilex kudingcha leaves 1, 2 1 1 1 2 1 ZUO Wen-jian , CHEN Hui-qin , LI Xiao-dong , WANG Zhen-hui , Dai Hao-fu , WANG Jin-hui 1. College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China 2. State Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China Abstract: Objective To investigate the chemical constituents in the leaves of Ilex kudingcha . Methods Repeated column chromatography with silical gel, maroporous resin column, Sephadex LH-20, and preparative HPLC were used to isolate the chemical constituents, and their structures were elucidated on the basis of spectral data. Results Eight compounds were isolated and identified as mateglycoside D (1), methyl caffeate (2), vanillin (3), gallic acid (4), caffeic acid (5), β-sitosterol (6), p -hydroxy-benzaldehyde (7), and ferulic acid (8). Conclusion Compounds 2 and 7 are first obtained from the plants of Ilex L., and compounds 1, 3, 4, and 8 are first obtained from I. kudingcha. Key words: Ilex kud