香椿子总多酚降血糖作用及机制分析-hypoglycemic effect of total polyphenols from toona sinensis and its mechanism analysis.docxVIP

PAGE PAGE III 基(O2·-)和烷氧基(LO·)和烷过氧基(LOO·)均有明显的抑制作用，且呈一定的量效关 系。香椿子多酚对羟自由基、超氧阴离子自由基、和抗脂质过氧化清除活性 SC50 值 分别为：0.290mg/ml、65.62μg/ml、 10.71μg/ml，比 Vit C 清除自由基能力弱，但是 总还原能力强于 Vit C。 (3) 香椿子多酚能降低链尿佐菌素诱导的糖尿病小鼠的血糖，对正常小鼠血糖没 有降低作用。香椿子多酚能降低糖尿病小鼠肝脏脏器指数，增加糖尿病小鼠肝糖原含 量，可能机制为降低肝脏 MDA 含量，增加 SOD 含量。 （4）：与模型组相比，香椿子多酚能够改善糖尿病大鼠症状，对糖尿病大鼠体重 没有显著增高；降低 FBG、GSP；提高 INS 和 C 肽含量；降低 MDA、NO 含量，提 高 SOD 含量；改善大鼠胰岛形态，提示香椿子多酚对胰腺有保护作用；香椿子多酚 不能降低 ALT 及 AST 含量；高剂量组能显著降低大鼠肝脏脏器指数，改善大鼠肝脏 形态。 结论：香椿子多酚具有良好的抗氧化作用；能降低糖尿病小鼠血糖水平，降血糖 机制可能与降低肝脏 MDA 含量，提高 SOD 含量有关；香椿子多酚能降低糖尿病大 鼠 FBG、GSP；提高 INS 及 C 肽含量；对糖尿病大鼠肝脏有保护作用；其降血糖作 用机制可能与抗氧化、调节肝脏糖代谢及抑制胰岛细胞凋亡有关。 关键词：香椿子 总多酚 降血糖作用 Abstract Objective: The research aimed to study the hypoglyeemic effects and mechanism of Total Polyphenols from seeds of Toona sinensis (A. Juss) Roem（TSTP）. Methods（: 1）Toona sinensis seeds was sent to a round bottom flask which added with 70% ethanol for extraction and refined by AB-8 macroporous resin .Determine the content of polyphenol by means of Folin-Ciocalten colorimetry，.（2）The reducing power of total polyphenol extracts from the seeds of T. sinensis was measured by using reducing K3Fe （CN）6 method. The antioxidant activities of polyphenols from seeds of Toona sinensis (A. Juss) Roem were evaluated by ferric reducing antioxidant power assay, hydroxyl ( ·OH) free radical scavenging capacity assay ，superoxide anion (O2·) free radical scavenging capacity assay，LO· and LOO·peroxidation reaction of PUFA of yolk LDL induced by Fe2+ scavenging capacity assay.（3）Diabetic models were induced by once intraperitoneal injection of STZ（150mg/kg）. Then the blood glucose was detected after 72 hours. when hypoglycemic point 11.1mmol /L the rats model were successful.Diabetic mice were randomly divided into model group, Metformin group and high- ,middle-and low - doses of TSTP groups . All groups were fed for 14 consecutive days, and then the blood glucose were detected by glucose oxidizes in the fifteenth day. The organ index detected. SOD and MDA were