铁皮石斛多糖提取及其在酸乳中应用-extraction of dendrobium officinale polysaccharide and its application in yoghurt.docxVIP

铁皮石斛多糖提取及其在酸乳中应用-extraction of dendrobium officinale polysaccharide and its application in yoghurt.docx

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铁皮石斛多糖提取及其在酸乳中应用-extraction of dendrobium officinale polysaccharide and its application in yoghurt

PAGE PAGE IV Abstract C2. The optimum conditions were extraction temperature is 81℃,  extraction time is 88 minutes, PH value is 6.0, ratio of material to water is l:25, and e -xtraction times is 2. Under such situations, polysaccharides yield can be up to 13.31mg/ml,and the polysaccharide content was 26.76% ; dendrobium officinale polysaccharides has a certain reduction ability of free radicals.In the polysaccharides concentration ranging from 0.2 to 0.4mg/g, reducing power increased with the increasing of polysaccharides concentrations. Dendrobium officinale polysaccharides also has a certain ability to remove ?OH and DPPH. The IC50 (half inhibition rate) of removing ?OH was as many 64 times as Vc, and the IC50 of removing DPPH was about 80times, these co-nclusions showed that dendrobium officinale polysaccharides has a weak ability of removing ?OH and DPPH. The dendrobium officinale polysaccharides was added as an additive agent to the yoghurt. Through orthogonal test, the best ingredients formula was obtained as follows: 0.8% dendrobium officinale polysaccharides, 65% fresh milk, 5.5% white sugar; and the optimum fermented process was 2.4% inoculum concentration, 40℃ fermented temperature while 4.8h fermented time.The final product has a colour of milk white, beautiful flavor, moderate sweet and sour, delicate taste, and rich flavour. Key words: dendrobium officinale polysaccharides; extraction;antioxidant; yoghurt 目录 目录 目录 第 1章 引言 1 1.1 多糖的研究概况 1 1.1.1 多糖的介绍及其分类 1 1.1.2 多糖的功能 2 1.1.3 多糖提取 3 1.2 铁皮石斛研究概况 7 1.2.1 铁皮石斛栽培技术的研究 7 1.2.2 铁皮石斛化学成分 8 1.2.3 铁皮石斛的药理学研究 10 1.3 课题研究的目的及意义 12 1.4 主要研究内容 13 第 2 章 铁皮石斛多糖的提取 14 2.1 引言 14 2.2 材料与方法 14 2.2.1 实验原料 14 2.2.2. 主要试剂 14 2.2.3 主要仪器及型号 15 2.2.4 实验方法 15 2.3 实验结果与分析 18 2.3.1 多糖提取单因素实验结果分析 18 2.4 铁皮石斛多糖提取工艺的优化 20 2.4.1 Box-Behnken 试验设计 20 2.4.2 模型建立与方差分析 22 2.4.3 响应面分析及提取工艺优化 23 2.5 本章小结 26 第 3 章 铁皮石斛多糖的抗氧化研究 27 V VI 3.1 引言 27 3.2 材料与设备 27 3.2.1 试验材料与试剂 27 3.2.2 试验

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