特定聚合度魔芋低聚糖的筛选与制备技术分析-screening and preparation technology analysis of konjac oligosaccharides with specific polymerization degree.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文 II万方数据 II 万方数据 本研究不仅建立了一套降解魔芋葡甘聚糖的工艺，而且采用乙醇分离和膜分离 方法对降解产物进行了分离，得到了不同聚合度的低聚糖，并且比较了各个分离产 物对肠道有益微生物的促生长作用，得到了具有最佳效果的聚合度片段，能够对魔 芋葡甘低聚糖加工生产进行靶向性的指导。 关键词：魔芋低聚糖 发酵工艺 酶解工艺 膜分离 PAGE IV万方数据 PAGE IV 万方数据 Abstract Konjac （ Amorphophallus konjac ） is a perennial herbaceous herb containing high glucomannan so far. Konjac glucomannan has the ability to lower blood cholesterol and sugar level, to promote intestinal activity and immune function etc. However, the intrinsic viscosity of native KGM was found to be the highest among all of the polysaccharides and chemical properties such as unstable nature are limited the use of KGM in the food industry. The previous studies found that the hydrolysis production of konjac glucomannan not only has the function which konjac glucomannan has, but also has shown better probiotic functions than glucomannan. Meanwhile konjac oligosaccharides have small molecular weight, physical and chemical properties, so it can be widely used in the food industry. The main results are shown as follows: （1）The effects of several variables on fermentation in flask with the strain Bacillus subtilis. was studied. The optimum medium ingredients for β-mannanase production in flask were as follows: Konjac flour（3%, w / v）, peptone（0.6%, w / v）, NaCl（1%, w / v）, K2HPO4（0.25%, w / v）, MgSO4（0.1% , w / v）, initial pH 5.8 for the medium, and that the optimum fermentation conditions was for 24 h at 37 ℃ at 200 rpm. With these conditions, the maximum β-mannanase activity reatched 74 U/ml medium which is 2.64 times of origin medium（28 U/ml）. （ 2 ） The hydrolytic conditions of konjak gum for producing glucomannan oligosaccharides were studied with high activity β-mannanase from Bacillus subtilis. The optimum hydrolytic conditions were as follows: konjak gum solution 140 g/L, hydrolytic temperature 50℃, β-mannanase dosage 50 U/g konjac gum, and hydrolytic time 4h. With these conditions, the rate of hydrolysis of konjac gum reached 25.9% . Meanwhi