26个香菇菌株遗传多样性分析.docVIP

26个香菇菌株的遗传多样性分析 　　摘要：香菇（Lentinus edodes）是世界上著名的食用菌之一，其菌株种类较多，遗传背景较为复杂。本研究利用ISSR-PCR技术，对26个香菇菌株进行DNA指纹分析，并使用NTSYS-PC软件对其遗传差异性进行聚类分析。结果表明，从106个随机引物中共筛选到9个引物，利用这9个引物对26个香菇菌株的DNA进行ISSR-PCR，检测到124个较为清晰的扩增位点，其扩增片段DNA分子量大小主要介于0.2～4.5 kb之间。通过聚类分析，发现相似水平在55%时出现种系分离；相似水平65%时，26个菌株聚为5个群组。本研究为山东省香菇遗传信息库的建立和遗传育种工作奠定了一定的基础。 　　关键词：香菇；ISSR-PCR；聚类分析；遗传多样性 　　中图分类号：S646.1+20.1文献标识号：A文章编号：1001-4942（2017）11-0020-05 　　Analysis of Genetic Diversity of 26 Lentinus edodes strains 　　Ren Haixia1， Gong Zhiyuan1， Jiang Zhiqin2， Lu Yurong2， Ren Pengfei1， Wan Luzhang1 　　（1.Institute of Agriculture Resources and Environment， Shandong Academy of Agricultural Sciences/ 　　Key Laboratory of Wastes Matrix Utilization， Ministry of Agriculture， Jinan 250100，China； 　　2. Bazhou Science and Technology Development Exchange Center of Xinjiang， Bazhou 841000，China） 　　AbstractLentinus edodes is one of the most famous edible fungus species in the world. It has various strains and complex genetic background. In this study， we conducted DNA fingerprinting of 26 familiar Lentinus edodes strains by ISSR-PCR， and analyzed their genetic diversity by cluster analysis of NTSYS-PC software. The results showed that， 9 primers were selected from 106 random primers， which were used to conduct DNA ISSR-PCR in 26 Lentinus edodes strains， and 124 relatively clear sites were amplified with the DNA molecular weight ranging from 0.2 kb to 4.5 kb. Cluster analysis results showed that， species separation was appeared at the similarity level of 55%， and the 26 strains were mainly divided into five groups at the similarity level of 65%. This study laid certain foundations for establishing the genetic information library and genetic breeding of Lentinus edodes in Shandong Province. 　　KeywordsLentinus edodes；ISSR-PCR；Cluster analysis；Genetic diversity 　　香菇（Lentinus edodes）?儆谡婢?门，担子菌纲，伞蘑目，皮伞科，富含蛋白质、多种人体必需氨基酸、糖类、矿物质、维生素和脂类等营养物质，同时含有香菇多糖、香菇嘌呤、双链核糖核酸等免疫活性物质，具有极高的营养价值[1，2]。香菇是世界上第二大食用菌栽培菌种，其产量仅次于双孢菇。中国香菇栽培生产发展迅速，近年来在香菇的产销量上一直位居世