COPD大鼠动物模型方法比较.docVIP

COPD大鼠动物模型方法比较 　　摘要：目的：探讨用于建立COPD大鼠动物模型较为理想的方法。方法： 对照I组大鼠对其进行30天饲养；对照II组大鼠对其进行3个月饲养；综合组大鼠COPD动物模型建立方法为熏香烟与气管注射内毒素相结合的综合方法；单纯组大鼠采用单纯熏香烟方法建立动物模型。将综合组与对照I组COPD大鼠动物模型、单纯组与对照II组COPD大鼠动物模型肺功能以及血气检测结果比较，并给予统计学分析，得出结论。结果：综合组与单纯组COPD大鼠动物模型毛发无光泽、神情呆滞，对照I组与对照II组COPD大鼠动物模型毛发有光泽、神情正常；综合组与对照I组COPD大鼠动物模型、单纯组与对照II组COPD大鼠动物模型相比较体重无显著性差异；综合组与对照I组COPD大鼠动物模型在Re、Ri、MVV、FEV .3、PaCO 、PaO 、SaO 等方面检测结果对比差异显著；单纯组与对照II组COPD大鼠动物模型在MVV、FEV .3、PaCO 等方面检测结果对比差异显著。结论：选用熏香烟与气管注射内毒素相结合的综合方法进行COPD大鼠动物模型建立，能够更好的复制COPD患者病情，从而为临床研究COPD的发病机制以及有效治疗方法提供更为可靠的依据，值得今后实验中推广应用。 　　关键词：COPD；大鼠；动物模型 　　COPD rat animal model comparison 　　Zeng Jianhong Huang Jianying Zeng Jun Hu Guangfen 　　Abstract：Objective： To explore for the establishment of COPD rat animal model of ideal methods. Methods： the control group I rats for 30 days of feeding； the control group II rats for 3 months feeding； integrated group COPD rat animal model for smoked cigarettes and tracheal injection of endotoxin in combination method； simple rats using simple method to establish animal model of cigarette smoke. The integrated group and the control group I COPD rat animal model， simple group and the control group II COPD rat animal model of pulmonary function and blood gas testing results， and give statistics analysis， draw the conclusion. Results： group and simple group COPD rats animal model hair mats， glassy， compared with the I group and the control group II COPD animal model of rat hair shiny， was normal； integrated group and the control group I COPD rat animal model， simple group and the control group II COPD rat animal model comparison no significant differences in weight； integrated group and the control group I COPD rat animal model in Re， Ri， MVV， FEV0.3， PaCO ， PaO ， SaO and other aspects of testing results showed significant differences； simple group and the control group II COPD rat animal model in MVV， FEV0.3， PaCO and detection results significantly. Conclusion： the selection of smoked cigarettes and tracheal injection of endo