hMSH2p53及PTEN在宫颈癌组织中表达及意义.docVIP

hMSH2p53及PTEN在宫颈癌组织中表达及意义 　　[摘要]目的：探讨宫颈癌组织中hMSH2、p53及PTEN蛋白表达的关系及意义。方法：采用免疫组织化学SP法检测54例宫颈癌中的hMSH2、p53及PTEN蛋白表达。结果：①宫颈癌组织中，hMSH2、p53蛋白阳性表达率分别为62.96%、66.67%，PTEN蛋白缺失表达率55.56%。②在hMSH2阳性表达的宫颈癌患者中，p53蛋白阳性表达率为76.47%、PTEN蛋白缺失表达率为52.94%。hMSH2与p53表达呈正相关（P＜0.05）。 结论：宫颈癌组织中，p53基因突变与错配修复基因的功能缺陷有关。 　　[关键词] 宫颈癌；hMSH2；P53；PTEN；免疫组织化学 　　[中图分类号] R737.3 [文献标识码]A [文章编号]1674-4721（2009）05（a）-014-02 　　 　　Expressions of hMSH2、p53 and PTEN in cervical cancers and its significance 　　SU Hong1，LI Mei2, WANG Xiuying3, LV Shen2* 　　(1.Department of Gynaecology and Obstetrics, the Second Affiliated Hospital, Dalian Medical University, Dalian116027, China； 2. Central Laboratory, the Second Affiliated Hospital of Dalian Medical University, Dalian116027, China；3. Department of Gynaecology and Obstetrics, Dalian Maternity Hospital，Dalian116021, China) 　　[Abstract] Objective: To disscuss the relationship ofhMSH2、p53 and PTEN protein expression in cervical cancers and its significance. Methods: Immunohistochemistry was used to examine the proteins expression of hMSH2，p53 and PTEN in 54 cases of cervical cancers. Results: ①In cervical cancer, the positive expression rates of hMSH2 and p53 protein were 62.96% and 66.67%. The lack expression rate of PTEN is 55.56%. ②In the cervical cancer cases of hMSH2 positive expression, the positive expression rate ofp53 and the lack expression rate of PTEN were 76.47% and 52.94%. hMSH2 and p53 protein expression was positively correlated (P0.05). Conclusion: Interaction between mismatch repair gene defect and p53 gene mutation may be related to the carcinogenesis of cervical cancer. 　　[Key words] Cervical cancer；hMSH2；p53；PTEN；Immunohistochemistry 　　 　　宫颈癌是最常见的妇科恶性肿瘤之一，在全球妇女恶性肿瘤中居第二位，其发病机制目前尚不清楚。目前认为，肿瘤是细胞多种基因突变累积的结果。这些基因突变主要发生在原癌基因、抑癌基因和DNA错配修复基因， hMSH2是第一个被发现的人类错配修复基因。p53已经证实是与人类肿瘤相关性最高的基因[1]。PTEN基因(phosphate and tension homology deleted on chromosome ten，PTEN)是一个具有磷酸酶活性的抑癌基因。本研究通过免疫组织化学法检测54例宫颈癌的hMSH2、p53及PTEN蛋白表达，探讨宫颈癌中hMSH2表达与p53、PTEN的关系及意义。