TregIL10IL6对狼疮性肾炎影响.docVIP

TregIL10IL6对狼疮性肾炎影响 　　【摘要】目的 检测活动期、静止期狼疮性肾炎（lupus nephritis，LN）患者及正常人外周血IL-10、IL-6、CD4+CD25+CD127lo调节性T细胞（Tregs）水平，探讨Treg、IL-10 、IL-6对狼疮性肾炎的的影响。方法 采用三色荧光染色通过流式细胞仪(FCM)分析外周血中CD4+CD25+CD127lo细胞水平，用CBA 流式蛋白分析系统检测外周血中IL-10、IL-6水平。结果 1、活动期LN患者外周血Tregs低于静止期（P0.05）；活动期LN患者外周血Treg低于正常组（P0.01）；静止期LN患者外周血Treg低于正常组（P0.05）。2、活动期LN患者 IL-10、IL-6与静止期相比显著升高（P＜0.01）。3、LN患者Treg与IL-10、IL-6、SLEDAI之间呈负相关（P0.05）。4、LN患者外周血IL-10、IL-6与SLEDAI呈正相关（P0.01）。结论 1、LN患者外周血中Treg及IL-10水平的变化，可作为狼疮活动性的指标。2、LN患者外周血中Treg与IL-10、IL-6呈负相关。3、Treg、IL-10、IL-6水平的变化参与了狼疮性肾炎的发病。4、激素有助于提高Treg的水平。 　　【关键词】系统性红斑狼疮 流式细胞术 调节性T淋巴细胞 IL-10IL-6 　　中图分类号：R593.242文献标识码：A文章编号：1005-0515（2011）2-001-03 　　 Treg, IL-10, IL-6 effects on lupus nephritis 　　Wang Wenhua Zhen Chuansheng Chen Jing WuWenhong LiWei 　　（The frist Affiliated Hospital of Fujian Medical University，Fuzhou 350005） 　　【Abstract】Objective To explore the role of CD4+CD25+CD127loregulatory T cells and IL-10 in the pathogenesis of lupus nephritis,we detect the level of IL-10、IL-6, CD4+CD25+CD127lo regulatory T cells(Tregs) in the peripheral blood ofpatients with lupus nephritis distinguishedactive stage and inactive stage.Methods 55 patients with lupus nephritis were differentiated according tovarious disease stage (active stage N=28, inactive stage N=27 ),and 21 cases of healthy volunteers were enrolled. Three-colour flow cytometry was used to quantitate proportions of Treg cells,and the level of IL-10、IL-6 in the peripheral blood were detected by Cytometric Bead Array System.Results 1. Compared with the inactive stage of patients with lupus nephritis， the level of CD4+CD25+CD127lo Tregs from patients with active LN was lower（P0.05）,as well ascompared the normal controls（P0.01）; compared with the normal controls , the level of CD4+CD25+CD127lo Tregs from LN inactive stage patients was lower（P0.05）. 2. Compared withthe inactive stage of LN patients , the level of IL-10、IL-6 was higher（P0.01）.3. The level of CD4+CD25+CD127lo T cell was correlated negatively with