TWEAK和Fn14在子宫内膜异位症发病中作用.docVIP

TWEAK和Fn14在子宫内膜异位症发病中作用 　　[摘要]目的 探?肿瘤坏死因子样凋亡弱诱导剂（TWEAK）和成纤维细胞生长因子诱导型14（Fn14）在子宫内膜异位症（EMs）中的表达与功能。方法 选取2014年6月～2015年6月我院妇产科收治的50例EMs患者作为研究对象，其中30例为异位囊壁组，20例为在位内膜组，同时收集与研究组匹配的因子宫肌瘤、宫颈病变等疾病接受全子宫切除术的20例患者作为对照组。利用免疫组织化学、Western-Blot及RT-PCR检测TWEAK、Fn14在不同组的基因和蛋白表达，根据所得数据分析TWEAK、Fn14表达水平与CA-125水平、抗子宫内膜抗体（EMAb）、修正子宫内膜异位症分期（r-AFS）的相关性。结果 三组患者的TWEAK、Fn14阳性表达率比较，差异有统计学意义（P0.05）；异位囊壁组、在位内膜组患者的TWEAK、Fn14阳性表达率均明显高于对照组，差异有统计学意义（P0.05）；EMs患者TWEAK、Fn14阳性表达率在r-AFS、CA-125、EMAb中差异有统计学意义（P0.05）；TWEAK、Fn14表达水平与r-AFS、CA-125、EMAb均成正相关（P0.05）。结论 TWEAK/Fn14参与子宫内膜异位症的发生和发展，TWEAK/Fn14水平异常可导致子宫内膜细胞的凋亡水平下降。 　　[关键词]子宫内膜异位症；TWEAK；Fn14；免疫组织化学 　　[中图分类号] R730.5 [文献标识码] A [文章编号] 1674-4721（2017）10（b）-0065-04 　　[Abstract]Objective To investigate the expression and function of tumor necrosis factor like apoptosis inducer （TWEAK） and fibroblast growth factor inducible 14 （Fn14） in endometriosis （EMs）.Methods 50 EMs patients in department of obstetrics and gynecology of our hospital from June 2014 to June 2015 were selected as the subjects.Among them，30 cases were ectopic cyst wall group，20 cases were eutopic endometrium group.At the same time，20 patients who received hysterectomy in the study group matched with the diseases of uterine fibroids and cervical lesions were collected as the control group.Gene and protein expression in different groups by immunohistochemistry，Western-Blot and RT-PCR detection of TWEAK，Fn14，according to the data analysis of TWEAK，Fn14 expression level and CA-125 level，anti endometrial antibody （EMAb），modified endometriosis stage （r-AFS） correlation analysis.Results The positive rates of TWEAK and Fn14 were significantly different between the three groups，the differences were statistically significant （P0.05）.The positive rates of TWEAK and Fn14 in ectopic cyst wall group and eutopic endometrium group were significantly higher than those in the control group，the differences were statistically significant （P0.05）.The positive rates of TWEAK and Fn14 in patients with EMs were