T―淋巴细胞酶联免疫斑点法检测在结核性结节性红斑中诊断意义.docVIP

T―淋巴细胞酶联免疫斑点法检测在结核性结节性红斑中诊断意义 　　DOI：10.16662/j.cnki.1674-0742.2017.23.046 　　[摘要] 目的 探讨T-淋巴细胞酶联免疫斑点法检测在结核性结节性红斑中的诊断意义。方法 2014年1月―2016年12月选取临床中的结节性红斑患者，行PPD皮试阳性（10 mm），进行T淋巴细胞酶联免疫斑点法（T-SPOT.TB）检测，根据T-SPOT.TB的检验结果，进行分组治疗，以及是否对抗结核治疗有效，共得到4组数据。分别选取T-SPOT.TB在不同阳性值时，来对4组数据进行统计分析，应用敏感性、特异性、阳性预测值和阴性预测值描述T-SPOT.TB检测在诊断结核性结节性红斑中的作用价值。结果 当设定T-SPOT.TB10为阳性时，T-SPOT.TB的灵敏度、特异度、阳性、阴性预测值分别为92.50%、41.18%、78.72%、70.00%；当设定T-SPOT.TB20为阳性时，分别为82.50%、70.59%、86.84%、63.16%。结论 在临床上对疑似结核引起的结节性红斑患者行T-SPOT.TB检测对鉴别诊断是有帮助的，T-SPOT.TB的阳性值越高，越支持结核感染。 　　[关键词] 结节性红斑；结核；诊断 　　[中图分类号] R529 [文献标识码] A [文章编号] 1674-0742（2017）08（b）-0046-03 　　Detection of T-lymphocyte Enzyme-linked Immunospot Assay in Tuberculous Nodular Erythema 　　CHENG Feng-gang1， TAN Yao-ju2， HUANG Yan-jun2， ZHANG Yan-bin3， XU Qi3 　　1. Department of Dermatology， Guangzhou Chest Hospital， Guangzhou， Guangdong Province， 510095 China； 2.Department of Laboratory， Guangzhou Chest Hospital， Guangzhou， Guangdong Province， 510095 China； 3.Department of Internal Medicine， Guangzhou Chest Hospital， Guangzhou， Guangdong Province， 510095 China 　　[Abstracts] Objective This paper tries to study the value of detection of T-lymphocyte enzyme-linked immunospot assay in tuberculous nodular erythema. Methods Patients with erythema nodosum from January 2014 to Dece mber 2016 were selected and treatment with positive PPD skin test （10 mm）， and （T-SPOT.TB） was detected， and according to the Results of T-SPOT.TB， the patients were grouped for treatment as well as whether it is effective， a total of four sets of data were obtained. T-SPOT.TB was used to analyze the four groups of data at different positive values， and the sensitivity， specificity， positive predictive value and negative predictive value were used to describe T-SPOT.TB in the diagnosis of tuberculous nodular. Results When T-SPOT. TB 10 was positive， the sensitivity， specificity， positive and negative predictive value were 92.50%， 41.18%， 78.72%， 70.00%. When the T-SPOT. TB value20 was posi