PKCNFκB信号传导途径在卵巢癌发生中作用.docVIP

PKCNFκB信号传导途径在卵巢癌发生中作用 　　摘要目的：探讨PKC-NF-κB信号传导途径调控卵巢癌细胞生长的机制，为通过阻断PKC-NF-κB信号传导通路抑制卵巢癌细胞生长，诱导肿瘤细胞凋亡,治疗卵巢恶性肿瘤提供新思路。实验材料与方法：采用免疫组织化学S-P法，对正常卵巢组织10例，卵巢上皮性良性肿瘤10例及卵巢上皮性癌49例组织中的PKCα，NF-κB进行检测。对检测结果进行统计分析。实验结果1.PKCα在卵巢上皮性良性肿瘤及卵巢上皮性癌组织中的阳性表达率分别为20.00%和59.18%，两者间的差异具有统计学意义（p0.05）。PKCα的表达强度与病理分级呈正相关（p0.05），与患者的年龄、临床分期及组织学分类均无关。2.NF-κB在卵巢上皮性良性肿瘤及卵巢上皮性癌组织细胞核中的阳性表达率分别为10.00％、69.39％，两者间的差异具有统计学意义（p0.05）NF-κB的表达强度与病理分级呈正相关（p0.05），与患者的年龄、临床分期及组织学分类均无关。3.卵巢上皮癌组织中PKC-α与NF-κB表达具有一致性，spearman等级相关系数r=0.643，p0.05。结论：1.卵巢上皮癌组织中PKCα与NF-κB表达均显著增高，且在不同病理分级有显著差异，低分化组阳性率明显高于高中分化组．2.PKCα与NF-κB在 卵巢上皮癌组织中的表达显著相关，两者协同表达。 　　关键词 蛋白激酶Cα；核因子κB；信号传导；卵巢癌；免疫组化 　　 　　Abstract Objective: We try to explore the effect of PKC-NF-ΚB signaling pathway on ovarian carcinoma cell and its mechanism. Our aim is to investigate in order to find the new methods for the tumor treatment. Methods: Selected wax lumps included 49 ovarian carcinoma,10 benign epithelial ovarian and 10 ovarian normal tissue to checkthe expression of PKCα and the NF-κB by the immunity organization S-P method. Results :1. The expression ratios of PKCα in benign epithelial ovarian and in ovarian carcinoma were 20.00% and 59.18% respectively, having significant difference (p0.01).The NF-кB expression level positively correlated with pathologic grade (p0.01),while no significant difference was observed in age，clinical stages and the histolopathology.2.The expression ratios of NF-κB in benign epithelial ovarian and in ovarian carcinoma were 10.00% and 69.39% respectively, having significant difference(p0.01).The NF-кB expression level positively correlated with pathologic grade (p0.01),while no significant difference was observed in age，clinical stages and the histopathology.3.There were statistically significant correlation among PKCα and NF-κB. Conclusion: There was an over expression of PKC and NF-κB in ovarian cancer. Their expression level positively correlated with pathologic grade. There was a significant correlation between the expressio