HBsAgHBcAg免疫荧光检测在HBVGN诊断中应用.docVIP

HBsAgHBcAg免疫荧光检测在HBVGN诊断中应用 　　[摘要] 目的 观察乙型肝炎病毒相关性肾炎(HBV-GN)患者肾脏组织中HBsAg、HBcAg的表达情况。方法 以50例HBV-GN患者为研究对象,另随机选择20例非乙型肝炎病毒相关性肾炎(NHBV-GN)患者作为对照组,用间接免疫荧光法检测肾穿刺活检组织HBsAg、HBcAg的表达。结果 HBV-GN患者肾组织中可检测HBsAg、HBcAg阳性颗粒,阳性率分别70%(35/50),24%(12/50);20例(NHBV-GN)患者肾脏组织HBsAg、HBcAg均未检出。与HBV-GN差异有统计学意义(P0.05)。结论 HBsAg、HBcAg免疫荧光标记是诊断HBV-GN的重要指标,HBV在肾组织内存在感染及复制,其在乙型肝炎病毒相关性肾炎的发病机制中具有重要作用。 　　[关键词] HBsAg;HBcAg;乙型肝炎病毒相关性肾炎;肾脏;免疫荧光 　　[中图分类号] R446.8[文献标识码] A [文章编号] 1673-9701(2010)04-12-03 　　 　　Application of HBsAg and HBcAg Immune Fluorescence Detection in Diagnosis of Hepatitis B Virus Associated Glomerulonephritis 　　CHEN Yupeng1CHEN Hong1WANG Xingfu1ZHANG Sheng1PENG Weihua2ZHENG Ke1 　　1.Department of Pathology, the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China; 2.Department of Nephrology,Fuzhou General Hospital of Nanjing Military Region,Fuzhou 350025,China 　　 　　[Abstract] Objective To observe the HBsAg and HBcAg expression in the renal tissue in hepatitis B virus associated glomerulonephritis(HBV-GN). Methods We selected 50 cases of HBV-GN as research group and the other randomly selected 20 cases of non-HBV-GN(NHBV-GN)as control group and used indirect immunofluorescence assay to detect the HBsAg and HBcAg expression in the renal puncture biopsies. Results The HBsAg and HBcAg-positive particles in the HBV-GN renal tissue could be detected,with the positive rates of 70%(35/50)and 24%(12/50),while HBsAg and HBcAg failed to be detected in the kidney tissue of 20 cases of NHBV-GN,with a significant difference between the two groups(P0.05). Conclusion The immune fluorescence detection of HBsAg and HBcAg is an important indicator in the diagnosis of HBV-GN. HBV plays an important role in the kidney tissue infection and replication and the pathogenesis of HBV-GN. 　　[Key words] HBsAg;HBcAg;Hepatitis B virus related glomerulonephritis;Renal tissue;Immunofluorescence 　　乙型肝炎病毒相关性肾炎(HBV-GN)是指乙型肝炎病毒感染人体后,病毒抗原HBsAg、HBcAg、HBeAg与体内相应抗体形成免疫复合物沉积在肾组织中,引起免疫复合物性肾小球肾炎[1