分子生物学课件之RNA的合成一.docVIP

分子生物学课件之RNA的合成一 本章重点：#8226；转录的反应体系；#8226；原核生物RNA聚合酶和真核生物中的RNA聚合酶的特点；#8226；RNA的转录过程；#8226；真核RNA的转录后加工，包括各种RNA前体的加工过程.本章难点：#8226；转录模板的不对称性及其命名；#8226；原核生物及真核生物的转录起始；#8226；mRNA前体的剪接机制；#8226；四膜虫rRNA前体的加工，核酶的作用机理。Introduction Transcription is the synthesis of asingle-stranded RNA from adouble-stranded DNA template.RNA synthesis occurs in the 5→3direction and its sequence corresponds to that of the DNA strand which is known as the sense strand.Transcription is catalyzed by an RNA polymerase which requires adsDNA template as well as the precursor ribonucleotides ATP,GTP,CTP and UTP.Coding strand of D