不同分化状态脐血间充质干细胞移植对脑梗死影响.docVIP

不同分化状态脐血间充质干细胞移植对脑梗死的影响 　　【摘 要】目的 探讨不同分化状态脐血间充质干细胞（umbilical cord blood mesenchymal stem cells，UCB-MSCs）对脑梗死的治疗影响。方法 分离培养UCB-MSCs并构建大鼠脑梗死模型；利用脑立体定位仪经侧脑室分别注射原代培养的UCB-MSCs和经碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）诱导培养的UCB-MSCs进行移植治疗大鼠脑梗死模型。免疫组化观察移植后的UCB-MSCs在脑梗死区分布及脑梗死周围组织胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）与神经元特异性烯醇化酶（neuron-specific enolase，NSE）的表达；同时，在细胞移植后的3d、7d、14d和28d对大鼠进行神经功能评价。结果 诱导后UCB-MSCs组大鼠脑梗死灶周围的GFAP、NSE阳性细胞数量及其神经功能评分在7d、14d、28d时间点均高于未诱导组。结论 诱导后的UCB-MSCs移植能对脑梗死大鼠模型缺血脑组织起到修复作用，并能促进脑梗死后大鼠神经功能改善。 　　【关键词】脑梗死；脐血间充质干细胞；碱性成纤维细胞生长因子；诱导 　　Effects of differentiated umbilical cord blood mesenchymal stem cells on cerebral infarction 　　Abstract：objective To explore the effection of differentiated umbilical cord blood mesenchymal stem cells（UCB-MSCs）on the treatment of cerebral infarction.Methods To isolate and culture UCB-MSCs and construct cerebral infarction model rats；Using brain stereotaxic instrument，primary cultured UCB-MSCs and cultured UCB-MSCs induced by basic fibroblast growth factor（bFGF）were respectively injected and transplanted into the lateral ventricles of the cerebral infarction model rats.By immunohistochemistry the distribution of the transplanted UCB-MSCs and the expression of glial fibrillary acidic protein（GFAP）and neuron-specific enolase（NSE）were observed separately in the cerebral infarction area.At the same time，the nervous function of the cerebral infarction model rats was evaluated after 3 days、7 days、14 days and 28 days.Results The positive expression of GFAP and NSE around the cerebral infarction area and the neurological function score in 7d、14d and 28d time point of the induced group were higher than the non-induced group.Conclusion The induced UCB-MSCs can repair the ischemia brain tissues of the cerebral infarction model rats and improve neural function of rats after cerebral infarction. 　　Key words：cerebral infarction；umbilical cord blood mesenchymal stem cells；fibroblast growth factors；induce 　　引 言 　　脑梗死后大脑梗