不同取材及处理方法对脂肪细胞影响研究.docVIP

不同取材及处理方法对脂肪细胞影响的研究 　　[摘要]目的：探讨不同取材及处理方法对脂肪细胞的影响。方法：随机选取临床行脂肪移植的就医者10名，在同一受术者双侧大腿前内侧分别用20ml注射器及吸脂机（吸脂负压60kPa）抽吸脂肪，吸脂针头直径均为2.5mm，将取出的脂肪经过静置及不同转速和不同时间的离心处理，采用完整脂肪细胞计数及组织学观察脂肪细胞的形态。结果：应用注射器法和吸脂机法吸出的脂肪经静置后，两组在完整脂肪细胞计数上无统计学差异，组织学也显示两组的脂肪细胞细胞壁均较完整；对比不同转速（1000转2min和3000转2min）完整脂肪细胞计数情况，分别为77%和74.5%，两者无统计学差异；对比不同离心时间（2000转1min和2000转3min），两组完整脂肪细胞比率分别为71%和68.7%，两组差异无统计学意义。组织学上看到，高的离心转速和长的离心时间相对于低的转速及短的离心时间而言，在脂肪细胞挤压变形及细胞壁受压情况要更加明显一些。离心后与离心前完整脂肪细胞计数无统计学差异，但组织学切片上仍可观察到离心后较离心前脂肪细胞有不同程度的变形。结论：在一定离心转速及离心时间范围内，离心与否以及不同转速与时间在脂肪细胞完整率上无明显差别，离心与静置相比以及高的离心转速和长的离心时间较低的离心转速和短的离心时间相比，脂肪细胞受压变形更明显一些。 　　[关键词]脂肪移植；脂肪细胞；组织学 　　[中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455（2014）22-1896-04 　　Rearch of the adipocyte injuries of different harvesting and processing methods. 　　ZHAO Yan-feng1，DONG Zhi-ming2，DONG Hong-hua1，TAO Hong-yu1 　　（1.Shijiazhuang Xingyuan Cosmetic Hospital， Shijiazhuang，Shijiazhuang 050000，Hebei，China； 　　2.Department of Pathology，The Fourth Peoples Hospital Affiliated to Hebei Medical University，Shijiazhuang 050000，China） 　　Abstract： Objective To research the influence to adipocyte of different obtain and processing method. Methods We have choose 10 patients that undergo fat transplantition，liposuction was done with syringe（20ml） and fat attacting machine（60kPa negative pressure ） from bilateral inner thigh respectively，diameter of liposuction needle was 2.5mm. Fat that gained was standing and then centrifugation by different rpm and time. The fat cells were evaluated under microscope and complete fat cell count. Results Ratio of complete fat cell was similar between syringe group and fat attacting machine group.To different centrifugal speed （1000rpm and 3000rpm）， ratio of complete fat cell was 77% and 74.5% respectively and has no statistical differences. To different centrifugal time （1min and 3min），ratio of complete fat cell was 71% and 68.7% respectively and has no statistical differences. But by histological observe， higher centrifugal speed and longer centrifugal time h