不同形态氮肥对油橄榄幼苗生长影响.docVIP

不同形态氮肥对油橄榄幼苗生长的影响 　　摘要：采用盆栽试验研究了不同形态氮肥对油橄榄（Olea europaea L.）幼苗叶片的叶绿素含量、可溶性蛋白质含量、可溶性糖含量、丙二醛（MDA）含量、过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性的影响。结果表明，在施用相同浓度的不同形态氮肥时，施用铵态氮肥对油橄榄幼苗的生长产生了一定的抑制作用，可溶性蛋白质含量低于施用硝态氮，可溶性糖含量降低，MDA含量显著增加，SOD和POD活性显著增加，CAT活性低于施用硝态氮，在一定程度上破坏了油橄榄幼苗的抗氧化系统，相比之下，施用硝态氮更有助于油橄榄幼苗的生长，延缓其衰老。 　　关键词：油橄榄（Olea europaea L.）；氮肥形态；抗氧化酶 　　中图分类号：S565.7 文献标识码：A 文章编号：0439-8114（2015）03-0650-04 　　DOI：10.14088/j.cnki.issn0439-8114.2015.03.036 　　Effects of Different Forms of Nitrogen on the Seedling Growth of Olea europaea L. 　　LIANG Jian1，XIE Wan-yan1，CAI Guang-ze1，SU Guang-can2 　　（1.School of Agricultural Science， Xichang College， Xichang 615013， Sichuan，China； 　　2.Liangshan Prefechure New Technical Development Co.， Ltd.， Xichang 615000， Sichuan，China） 　　Abstract： The effects of nitrogen forms on chlorophy content， soluble protein content，soluble sugar， MDA of seedling and three antioxidant enzymatic activities （SOD，POD，CAT） in the antioxidant system of Olea europaea L. were studied with same ration gradients of NH4NO3 and Ca（NO3）2 added to soil. The results showed that when applying the same concentration of different forms of nitrogen fertilizers， the administration of ammonium nitrogen fertilizer had a certain inhibitory on the growth of olive seedlings. Soluble protein content was lower than that of nitrate nitrogen treatment. Soluble sugar content decreased. Malondialdehyde， SOD and POD activity increased significantly. CAT activity was lower than that nitrate nitrogen treatment. Ammonium nitrogen fertilizer destroyed the antioxidant system of the olive seedling. Application of nitrate fertilizer was more conducive to olive seedlings with delaying its senescence. 　　Key words： Olea europaea L.；form of nitrogen fertilizer；antioxidant enzyme 　　油橄榄（Olea europaea L ）属木樨科常绿乔木，原产地中海沿岸地区，是一种油料植物[1]。橄榄油又被称为流动的黄金，它有很高的营养价值、药用价值，其美容功效、保健功能已被世人广为认同。近年来，随着人们生活水平及保健意识的提高，国内外对橄榄油的消费急剧增大，市场需求明显增长，从而带动油橄榄产业的发展。但由于油橄榄适生区域狭窄，限制了大规模种植油橄榄的可能性。油橄榄生长需要适宜的土壤、日照、湿度、光热等气候条件。 　　氮是限制