常用动物实验麻醉物对大鼠心血管系统的影响.docVIP

PAGE PAGE 13 常用动物实验麻醉药物对大鼠心血管系统的影响 孙安会1，谷捷1,2，吴涛1，袁肇凯*1，蔡雄2，胡志希1，简伟雄1，李鑫2 （1.湖南中医药大学；2.中医诊断学湖南省重点实验室，湖南 长沙 410007) 摘要：目的：探讨不同动物实验麻醉药物对大鼠心血管系统的影响。方法：采用BioPac MP150动态记录四种麻醉药物麻醉后大鼠的心电图、血压，采集麻醉后不同时间点大鼠的血糖以及实验终点肝肾功能、心肌酶、电解质。结果：水合氯醛可引起严重的室性心律失常，异氟烷对心率有抑制作用，戊巴比妥钠可引起大鼠心电图P波增高，乌拉坦可引起大鼠心电图J点抬高。乌拉坦、戊巴比妥钠均可引起SBP升高，水合氯醛可引CK升高，异氟烷可降低CK、CKMB，戊巴比妥钠、异氟烷有降低ALT、AST的作用，水合氯醛、戊巴比妥钠、异氟烷均可降低肌酐，四种麻醉药物均有降低血钠、血钾的作用。结论：水合氯醛对心血管系统的影响明显，不适合用于心血管病的动物实验研究，戊巴比妥钠、乌拉坦、异氟烷可适当选择作为心血管病的动物实验研究。 关键词：动物实验，麻醉药物，大鼠，心血管系统 The Impact of Anesthetic Drugs Commonly Used in Animal Experiments on Cardiovascular System of Rats Sun An-hui1，Gu Jie1,2，Wu Tao1，Yuan Zhao-kai1*，Cai Xiong2，Hu Zhi-xi1，JianWei-Xiong1，Li Xin2 (1.Hunan University of Chinese Medicine；2.Hunan Provincial Key Laboratory of Diagnostics in Chinese Medicine, Chagnsha, Hunan410007, China) Abstract:Objective: To explore the Impact of different anesthetic drugs commonly used in animal experiments on cardiovascular system of rats.Methods:Recording electrocardiogram(ECG) and blood pressure dynamically througy BioPac MP150 after anesthesia.In addition,collecting blood glucose at different time-points and hepatic function,kidney function,cardiac enzyme,electrolytes at the end of the test.Rusults:Chloral hydrate can cause severe ventricular arrhythmias.Isoflurane had inhibitory effect on heart rate.Pentobarbital Sodium can induce ECG P wave increased.Urethane can cause J point elevation of ECG. Blood pressure in urethane and pentobarbital sodium groups were higher.Chloral hydrate may cause CK to be raised,while isoflurane may have the opposite effect on CK and CKMB.Alanine aminotransferase and aspartate aminotransferase in pentobarbitol sodium and isoflurane groups were decreased.Creatinine in chloral hydrate, pentobarbital sodium and isoflurane groups were lower,and so does serum sodium, serum potassium in four groups.Conclusion: Chloral hydrate had obvious effects on the cardiovascular system,it was not suitable for animal studies on cardiovascular d