丙泊酚对大鼠单肺通气时代谢酶影响.docVIP

丙泊酚对大鼠单肺通气时代谢酶的影响 　　 【摘要】 目的：观察丙泊酚对大鼠单肺通气时肺代谢酶的影响，为丙泊酚在胸科麻醉中的应用提供实验研究的数据。方法：40只健康清洁级SD大鼠随机分成四组：P 1组：空白组(灌注液中无丙泊酚)；P2 组：含36 mg/L丙泊酚；P3 组：含24 mg/L丙泊酚；P 4组：含12 mg/L丙泊酚，每组10只。按随机顺序取大鼠进行实验，称量体重，麻醉，气管切开插管接呼吸机行单肺机械通气1 h。然后取出肺组织。-70 ℃保存，以备统一作Ca2+ -ATPase、Na+ -k+ ATPase测定。结果：肺组织Na+-K+ATPase活力：P1组通气侧活力显著低于P2、P3、P4 组通气侧(P0.01)；P1 组非通气侧活力显著低于P2、P3 、P 4组非通气侧(P0.01)。各组非通气侧均低于同组通气侧(P0.01或0.05)。肺组织Ca2+-ATPase活力：P1 组通气侧显著低于P2、P3、P4组通气侧(P0.01)；P1组非通气侧显著低于P2、P3、P4组非通气侧(P0.01)。各组非通气侧均显著低于同组通气侧(P0.01)。结论：通过对大鼠单肺通气时的灌注实验研究发现，丙泊酚对单肺通气时的肺代谢酶有保护作用，其程度与丙泊酚浓度在一定范围内无关。 　　 【关键词】 单肺通气； Na+-K+ATP酶； Ca2+ATP酶； 丙泊酚； 大鼠 　　 　　 The Effect of Propofol on Metabolic Enzyme of Rats During one-lung Ventilation/WANG Tong-chun，GAO Hong，ZHOU Jing，et al.//Medical Innovation of China，2012，9(13):006-007 　　 【Abstract】 Objective:To investigate the effect of propofol on metabolic enzyme of rats during one-lung ventilation.Methods:40 rats randomly divided into 4 groups(n=10，each):group P1 (no propofol in the perfusate )，group P2 (the concentration of propofol in perfusate was 36mg/L)，group P3(24 mg/L)，group P4 (12 mg/L). Metabolic enzyme was quantified after one-lung ventilation .After OLV finished ，lung tissue sample were harvest for the criterions of the Na+-K+ATPase activity，Ca2+ATPase activity.Results:(1)The Na+-K+ATPase activity: as compared to the values in group P2、group P3 and group P4 ventilated lung， the Na+-K+ATPase activity was low in group P1 ventilated lung(P0.01)； as compared to the values in group P2、group P3 and group P4airproof lung， the Na+-K+ATPase activity was low in group P1 airproof lung(P0.01)；as compared to the values in reciprocal group ventilated lung， the Na+-K+ATPase activity was low in each group airproof lung(P0.01 or 0.05).(2)The Ca2+-ATPase activity: as compared to the values in group P2、group P3 and group P4 ventilated lung， the Ca2+-ATPase activity was low in group P1 ventilated lung(P0.01)； as compared to the values in group P2 、group P3 and group P4 airproof lung， the Ca2+-A