乙型肝炎病毒pre S1与乙肝两对半和HBV DNA联合检测临床价值分析.docVIP

乙型肝炎病毒pre S1与乙肝两对半和HBV DNA联合检测的临床价值分析 　　[摘要]目的 分析乙型肝炎病毒前S1蛋白抗原（pre S1）、乙肝两对半、乙肝病毒基因（HBV DNA）联合检测在乙肝患者临床诊断中的应用价值。 方法 选取2011年4月～2014年4月我院住院及门诊收治的160例乙肝患者作为研究对象，于清晨空腹条件下取其血清标本，采用酶联免疫法检测血清pre S1及乙肝两对半水平，同时采用荧光定量扩增技术检测血清内HBV-DNA水平。 结果 大三阳组患者前S1抗原与乙肝HBV DNA阳性率均明显高于其他HBV-M模式，组间对比差异显著（P0.05），HBsAg阳性HBcAb阳性组前S1抗原阳性率明显高于小三阳组（P0.05）；前S1抗原阳性组HBV DNA阳性率明显高于对应前S1抗原阴性组，其中以HBsAg+HBeAg+HBcAb+pre S1+组HBV DNA阳性率最高，且其HBV DNA含量水平同样最高；146例HBsAg阳性患者中，137例HbeAg阳性，阳性率为93.8%，且其中HBeAg+组，前S1抗原阳性率为88.9%，明显高于HBeAg-组，组间对比差异显著（P0.05）；HBsAg阳性患者HBV DNA阳性100例，阳性率68.5%。且HBV DNA阳性组前S1抗原阳性率为85.0%，明显高于HBV DNA-组，提示HBsAg阳性患者血清中pre S1与HBV DNA相关性较强（P0.05）。结论 采取三项联合检测，可在尽早时间内发现乙肝患者病毒感染情况，为患者的诊断与治疗提供指导。 　　[关键词]乙型肝炎病毒；前S1抗原；HBV DNA；联合检测；价值 　　[中图分类号] R512.6 [文献标识码] B [文章编号] 2095-0616（2015）17-146-04 　　Clinical value analysis of combined detection of Hepatitis B Virus pre S1 protein antigen， five indexes of Hepatitis B and HBV DNA of patients with Hepatitis B in clinical diagnosis 　　YE Yuanqing LUO Wenshen HUANG Hua LIANG Hongmei CHEN Qun YANG Lianjun BAO Hongxia 　　Department of Laboratory， Second Peoples Hospital， Longgang District， Shenzhen 518112， China 　　[Abstract] Objective To analyze the application value of combined detection of Hepatitis B Virus（HBV） pre S1 protein antigen， five indexes of Hepatitis B and HBV DNA of Patients with Hepatitis B in Clinical Diagnosis. Methods 160 patients with Hepatitis B who were admitted to our hospital from April 2011 to April 2014 were selected as research subjects. Patients’ serum samples in fasting condition were collected in the morning. Serum pre S1 and five indexes of Hepatitis B were detected by enzyme linked immunoassay and level of HBV DNA in serum was detected by fluorescent quantitative PCR at the same time. Results Positive rates of pre S1 antigen and HBV DNA of patients in the great three positive group was all significantly higher than other HBV-M models. The compared groups have significantly differences （P 　　[Key words] Hepatitis B Virus； Pre S1 ant