乳腺癌患者外周血CK19 mRNA检测临床意义.docVIP

乳腺癌患者外周血CK-19 mRNA检测的临床意义 　　[摘要] 目的 探讨外周血CK-19 mRNA表达与乳腺癌的关系,评价其在乳腺癌诊断上的价值。方法 应用RT-PCR技术检测200例乳腺癌患者外周血中CK-19 mRNA表达情况,并以66例乳腺良性疾病和60例正常健康女性作为对照。结果 200例乳腺癌患者48例外周血CK-19为阳性,66例乳腺良性疾病患者中3例阳性,而同时检查的60位健康者,他们的外周血CK-19均为阴性;不同年龄病人的CK-19检测阳性率对比,彼此之间差异无统计学意义;肿瘤不同分期CK-19阳性率对比差异有统计学意义。结论 RT-PCR法检测CK-19 mRNA可作为乳腺癌诊断的指标。 　　[关键词] 乳腺癌; CK-19 mRNA; 逆转录聚合酶链反应(RT-PCR) 　　[中图分类号] R737.9 [文献标识码] A [文章编号] 1673-9701(2009)33-15-02 　　 　　Clinical Significance of CK-19 mRNA in Patients with Breast Cancer 　　MENG Fanjie WANG Yufang WANG Guofeng 　　Yidu Central Hospital of Weifang City,Qingzhou 262500,China 　　 　　[Abstract] ObjectiveTo investigate the clinical significance of CK-19 mRNA in patients with breast cancer. MethodsThe CK-19 mRNA expression in the peripheral blood of 20 cases of breast cancer was measured by using RT-PCR method before operation,and 66 patients with benign breast diseases and 60 healthy women were taken as controls. ResultsThe positive rates of CK-19 mRNA in patients with breast cancer and benign breast diseases were 24%(48/200)and 4.54%(3/66),respectively,and CK-19 mRNA was all negative in the 60 healthy women. Thepositive rate difference between the breast cancer group and the control group was significant(P0.01). There was no difference in the positive rate of CK-19 mRNA in patients of all ages. The positive rates at 0～I,II and III stages were 15.38%(8/52),20%(24/120)and 57.14%(16/28),respectively. There was a significant difference in the positive rate between stage III and stages of 0～I and II(P0.05). ConclusionCK-19 mRNA can be used as a molecular marker in the peripheral blood by method of RT-PCR for the detection of breast cancer. 　　[Key words]Breast cancer; Cytokeratin-19 mRNA; RT-PCR 　　 　　乳腺癌是一种全身性疾病,在疾病早期肿瘤细胞就可能经血液播散出现远处转移[1]。乳腺癌细胞为上皮来源的细胞,而外周血中主要是内皮细胞,而并没有上皮成分,因此乳腺癌患者如果外周血中存在肿瘤细胞,则其血液中会出现上皮细胞或上皮细胞脱落的组织成分。 　　本文通过RT-PCR方法检测乳腺癌患者外周血CK-19 mRNA表达,研究外周血CK-19 mRNA表达与乳腺癌的关系,并探讨其临床意义。 　　 　　1 材料与方法 　　 　　1.1 一般资料 　　为我院2006年8月至2008年7月女性乳腺癌手术患者200例共200侧,