人体血浆微小RNA―499对急性心肌梗死患者诊断价值.docVIP

人体血浆微小RNA―499对急性心肌梗死患者的诊断价值 　　[摘要] 目的 探讨人体血浆微小RNA-499（miRNA-499）对急性心肌梗死患者的诊断价值。 方法 选取本院2012年1～12月收治的60例急性心肌梗死患者作为观察组，另选择同期本院体检中心的60例无心脑血管疾病史的健康体检者作为对照组。以两组的miRNA-499为指标进行含量考察，比较急性心肌梗死患者的miRNA-499、cTn、CRP诊断符合率。 结果 观察组的miRNA-499相对表达量为3.623±1.123，显著高于对照组的0.819±0.017，差异有统计学意义（P0.05）。60例AMI患者中，miRNA-499的阳性率为100%，cTnI与cTnT的阳性率均为96.7%，CRP的阳性率为86.7%。 结论 miRNA-499可作为急性心肌梗死的新标志物，进一步提高急性心肌梗死诊断的特异性。 　　[关键词] 血浆微小RNA-499；急性心肌梗死；早期诊断 　　[中图分类号] R542.2+2 [文献标识码] A [文章编号] 1674-4721（2015）05（c）-0099-03 　　[Abstract] Objective To explore the diagnostic value of human plasma miRNA-499 for patients with acute myocardial infarction. Methods 60 patients with acute myocardial infarction from January 2012 to December in our hospital were selected as the observation group.60 cases with no history of cerebrovascular disease received health examination in the physical examination centre of our hospital were selected as the control group.The content of miRNA-499 in the two groups was investigated as the index，diagnostic accordance rates of miRNA-499，cTn and CRP in 60 patients with AMI was compared. Results The relative expression level of miRNA-499 in the observation group was 3.623±1.123，which was higher than 0.819±0.017 in the control group，with significant difference（P0.05）.In 60 patients with AMI，the positive rate of miRNA-499 was 100%，the positive rate of cTnI and cTnT was 96.7% respectively，and the positive rate of CRP was 86.7%. Conclusion MiRNA-499 can be used as a new marker of acute myocardial infarction.and can further improve the specificity of acute myocardial infarction diagnosis. 　　[Key words] Plasma miRNA-499；Acute myocardial infarction；Early diagnosis 　　目前，心血管疾病严重影响着人类健康，其中急性心肌梗死患者的病情最重，死亡率最高，及时准确地确诊急性心肌梗死可以缓解病情，降低死亡率[1-2]。临床用于诊断急性心肌梗死的主要标志物为血清超敏心肌肌钙蛋白I和心肌肌钙蛋白I（cTnI），但确诊时间较长[3-5]。本研究采用qRT-PCR检测方法对本院收治的急性心肌梗死患者进行miRNA-499含量动态检测，旨在进一步了解miRNA-499对AMI的诊断价值。 　　1 资料与方法 　　1.1 一般资料 　　选取本院2012年1～12月收治的60例急性心肌梗死患者作为观察组，其中男38例，女22例；年龄39～82 岁，平均（59.4±6.9）岁。另选择同期本院体检中心的60例无心脑血管疾病史的健康