土霉素对猪粪污染土壤微生物数量和酶活性影响研究.docVIP

土霉素对猪粪污染土壤微生物数量和酶活性影响研究 　　摘要研究外源土霉素对受不同养殖污染程度的土壤生物学性质的影响,结果表明:添加土霉素可降低土壤微生物数量,降低比例由低至高依次为:猪粪堆放处土壤、长期施用猪粪农田土壤、不施用畜禽粪农田土壤;添加土霉素对土壤脲酶和中性磷酸酶活性的抑制程度为:不施用畜禽粪农田土壤长期施用猪粪农田土壤猪粪堆放处土壤,影响大小随受畜禽养殖污染程度增加而减小。长期受养殖污染的土壤具有较高的土霉素抗性微生物水平,可能与养殖污染促进了土壤环境中抗生素抗性物质形成有关。 　　关键词畜禽养殖污染;土霉素;抗性;土壤微生物;酶活性 　　中图分类号 X171;S181 文献标识码A文章编号 1007-5739(2010)08-0290-03 　　 　　EffectResearchofOxytetracyclineon MicroorganismsNumberandEnzymeActivityinSoil AffectedbyPigManure 　　MA Wan-zhu 1ZHANG Ming-kui 2 * 　　(1 Institute of Environment Resources,Soil and Fertilizer,Zhejiang Academy of Agricultural Science,Hangzhou Zhejiang 310021; 2 Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition,College of Natural Resource and Environmental Sciences,Zhejiang University) 　　AbstractThe effect of oxytetrycycline on animal manure-impacted soils was studied in this paper. The results showed that the numbers of soil microorganisms could decrease by adding oxytetracycline into the soil. The microorganisms number decreased in the order was pig manure storage site,vegetable field with long-tern application of pig manure,and vegetable field without any organic manure. The inhibited effect of oxytetracyclineon either neutral phosphotase activity or urease activity as well as numbers of microorganisms increased in the sequence as soil from pig manure storage site,soil from vegetable field with long-tern application of pig manure,soil from vegetable field without any organic manure. The effects decreased with the increasing of pollution of soil with pig manure. It indicated that oxytetrycycline antibiotic resistance levels of soil microorganisms increased with increasing influence of pig manure,which may be related with the formation of antibiotic substance prouduced by the cultivation pollution. 　　Key wordslivestock production pollution;oxytetracycline;antibiotic resistence;soil microorganism;enzyme activity 　　 　　畜禽养殖业中抗生素的广泛应用大大降低了动物疾病的发生,促进了规模化养殖业的发展。目前,人类临床治疗和畜牧业使用抗生素各约一半,每吨饲料中抗生素添加量一般在3～220 g之间[