基于ARM双色近红外荧光扫描系统.docVIP

基于ARM双色近红外荧光扫描系统 　　摘 要： 由于?呙柩?品的多样化，样品的发光点并不一定在焦平面上；双色近红外荧光扫描分析可以实现数据对比以及准确的量化分析。因此，设计基于激光共聚焦原理的ARM控制、三维移动、双色近红外荧光扫描的系统。采用STM32F407作为主控板，THB7128芯片作为x，y，z三轴步进电机的驱动，可以实现快速定位和精准扫描。信号采集电路由H11461?03型号的侧边PMT，以OPA686芯片作为运放的电流?电压转换和放大电路，LM747H芯片构造的二阶巴特沃斯低通滤波电路以及ADS8320采样电路组成。系统的背景噪声实验结果显示信号范围在0.075～0.2 V之间，且数值较低、变化平稳。 　　关键词： 双色近红外荧光扫描； 激光共聚焦； ARM处理器； 三维移动； 信号采集 　　中图分类号： TN219?34； TN24； TM933 文献标识码： A 文章编号： 1004?373X（2017）18?0139?04 　　Two?color near?infrared fluorescence scanning system based on ARM 　　ZHU Nannan1，2， WANG Bidou2， LUO Gangyin2 　　（1. School of Communication and Information Engineering， Shanghai University， Shanghai 200444， China； 　　2. Suzhou Institute of Biomedical Engineering and Technology， Chinese Academy of Sciences， Suzhou 215163， China） 　　Abstract： The diversification of scanning samples makes the light?emitting point can′t locate on the focal plane. Two?color near?infrared fluorescence scanning analysis can realize data comparison and accurate quantitative analysis， therefore a two?color near?infrared fluorescence scanning system with three?dimensional movement and ARM control based on the principle of laser confocal scanning was designed. The STM32F407 is taken as the main control board and THB7128 is taken as the driver of x?y?z?axis stepper motor to realize the fast positioning and accurate scanning. The signal acquisition circuit is composed of the parasitic PMT of H11461?03 type， OPA686 operational amplifier chip as current?voltage conversion and amplification circuit for operational amplification， second?order Butterworth low?pass filter circuit constructed with LM747H， and ADS8320 sampling circuit. The experimental results of system background noise show that the signal voltage is within 0.075～0.2 V， and its value is relatively low and stable. 　　Keywords： two?color near?infrared fluorescence scanning； confocal laser； ARM processor； three?dimensional movement； signal acquisition 　　近年来，生物芯片扫描技术在生命科学、微电子、化学等领域广泛使用。近红外荧光采用荧光标记技术，其染料具有独特性，发射