基于组分结构理论对丹皮赤芍和白芍化学成分对比研究.docVIP

基于组分结构理论对丹皮赤芍和白芍化学成分对比研究 　　[摘要]基于“组分结构”理论对丹皮、赤芍与白芍化学成分进行分析比较。采用HPLCDAD技术，分析了13批丹皮，14批不同产地的赤芍与10批不同产地的白芍药材，并运用聚类分析，主成分分析等分析方法进行分析；利用F检验考察了丹皮、赤芍与白芍主成分间的显著性差异程度。建立了13批丹皮、14批赤芍与10批白芍药材的高效液相色谱指纹图谱，指认了7个萜苷类和酚酸类成分，根据系统聚类分析、主成分分析的结果，并结合“组分结构”理论对丹皮、赤芍与白芍进行对比研究。丹皮、赤芍与白芍功效不同，通过试验比较研究丹皮、赤芍与白芍中的主要化学成分的质量分数及其配比，发现存在显著性差异。 　　[关键词]丹皮；赤芍；白芍；指纹图谱；主成分分析；组分结构 　　[Abstract]To analyze and compare the chemical compositions of Moutan Cortex， Paeoniae Rubra Radix and Paeoniae Alba Radix based on component structure theory Thirteen batches of Moutan Cortex， 14 batches of Paeoniae Rubra Radix from different origins and 10 batches of Paeoniae Alba Radix from different origins were analyzed by HPLCDAD method Hierarchical cluster analysis and principal component analysis were used for analysis The significant differences of principal component from Moutan Cortex， Paeoniae Rubra Radix and Paeoniae Alba Radix were investigated by using F test HPLC fingerprints were established for 13 batches of Moutan Cortex， 14 batches of Paeoniae Rubra Radix and 10 batches of Paeoniae Alba Radix， and 7 glycosides and phenolic acids components were identified Comparative study of Moutan Coetex， Paeoniae Rubra Radix and Paeoniae Alba Radix was conducted according to the results of hierarchical cluster analysis， principal component analysis and component structure theory Moutan Cortex， Paeoniae Rubra Radix and Paeoniae Alba Radix have significant differences in mass fraction of major chemical components and their ratios， leading to different curative effects 　　[Key words]Moutan Cortex； Paeoniae Rubra Radix； Paeoniae Alba Radix； fingerprint； component structure； principal component analysis 　　doi：10.4268/cjcm 　　丹皮、赤芍与白芍均是临床上常用的中药。丹皮为毛茛科植物牡丹皮Paeonia suffruticosa Andr的干燥根皮，具有清热凉血，活血化瘀功效[1]。赤芍为毛茛科植物芍药P. lactiflora Pall或川赤芍P. veitchii Lynch的干燥根，具有清热凉血，散瘀止痛功效[1]。白芍为毛茛科植物芍药P. lactiflora Pall的干燥根，具有养血调经，敛阴止汗，柔肝止痛，平抑肝阳功效[1]。2015年版《中国药典》中含有丹皮的中成药共计79余种，如活血止痛膏、养阴清肺丸、六味地黄丸等；含有赤芍的中成药共计89种之余，如血府逐淤口服液、脑心通胶囊、少腹逐瘀丸等；