毕业论文(设计)-《白腐菌预处理秸秆优化条件的筛选》.docVIP

华中科技大学 生命科学与技术学院 摘要  预处理技术是纤维质乙醇生产的主要技术瓶颈之一，生物预处理技术因具有低能耗和环境友好的优势而受到广泛关注。白腐菌是目前已知的降解木质素最强的微生物.但白腐菌直接对秸秆进行预处理所得到的糖化率和乙醇的产量并不高，为了显著提高白腐菌预处理秸秆所得的糖化率，对白腐菌预处理秸秆的条件进行了优化，通过改变它的培养温度、培养时间、是否通氧以及培养基中K、Mn、Ca、Cu等离子的添加，采用分光光度计计算测定其糖化率，得到优化的因子为28℃温度条件下培养4周，通氧，添加低浓度Mn离子，提供更高的培养基湿度。 关键字：白腐菌 ； 预处理秸秆 ；优化 ；糖化率  Abstract Pretreatment, during the process of fuel ethanol production from lignocellulose, is one of the main technical bottleneck. Because of reduced energy and less pollution, biological pretreatment attracts a wide spread attention. The biological degradation of lignin in corn straw with white rot fungi is the most effective fungi among Microbe .But the directly degradation of white rot fungi has low saccharification and fuel ethanol on the corn .To improve the saccharification distinctly , we researched the influence of different culturing conditions of white rot fungus on the pretreatment of corn .We designed different culturing temperature and time and to add water oxygen 、K、Mn、Ca、Cu or not to culture white rot fungi .And the we measured the saccharification through spectrophotometer .At last we concluded that the most effective factor on the pretreatment of corn is to culture 4 weeks in the 28℃ environment with adding oxygen and Mn with a higher humidity . Key Words: white rot fungi pretreatment optimization saccharification rate 目录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc2820811271 引言 PAGEREF _Toc282081127 \h 1 HYPERLINK \l _Toc2820811281.1 秸秆的利用现状和结构特征 PAGEREF _Toc282081128 \h 1 HYPERLINK \l _Toc2820811291.2 木质纤维素的结构和组成 PAGEREF _Toc282081129 \h 1 HYPERLINK \l _Toc2820811301.3 木质纤维素的酶解糖化 PAGEREF _Toc282081130 \h 2 HYPERLINK \l _Toc2820811311.4 纤维质乙醇的研究及其存在的技术屏障 PAGEREF _Toc282081131 \h 3 HYPERLINK \l _Toc2820811321.5 秸秆预处理技术 PAGEREF _Toc282081132 \h 3 HYPERLINK \l _Toc2820811331.5.1 理化预处理工艺 PAGEREF _Toc282081133 \h 3 HYPERLINK \l _Toc2820811341.5.2 生物预处理工艺 PAGEREF _Toc282081134 \h 4 HYPERLINK \l _Toc2820811351.6 白腐菌简介 PAGEREF _Toc282081135 \h 4 HYPERLINK