SP对大鼠DRG神经元膜NMDA和SP自身受体表达的调制-生理学专业论文.docxVIP

II II Abstract Part 1. The effects of SP on the behavior in capsaicin-induced pain in rats. Objective: In this study, we made the neuropathic pain model through injecting capsaicin around the dorsal thigh sciatic nerve in rats, then gave exogenous SP by intraperitoneal injection to explore the effects of SP on thermal hyperalgesia in capsaicin-induced pain in rats. Methods: To investigate these effects, 48 SD rats were randomly divided into 6 groups: Group A: normal control group; Group B: capsaicin group; Group C: capsaicin 10-4 mol/L +SP 10-5 mol/L group; Group D: intraperitoneal injection of Capsaicin 10-4 mol/L +SP 10-4 mol/L injection; Group E: intraperitoneal injection of SP 10-3 mol/L + Capsaicin 10-4 mol/L injection; Group F: intraperitoneal injection of normal saline. Each group was done with hot-plate test with the duration of 10, 20, 30, 40, 50, 60 min before and after experiment. Results:(1) The rats were significantly shortened after injection of capsaicin (P0.05). (2) After SP was given in capsaicin-induced pain rats as an intervention, the rats were significantly shorter than the single injection of capsaicin (P0.05). Conclusion: According to experimental results, SP may play a role in promoting nociceptive pain information in rats and resulting in hyperalgesia by combining its receptors. Part 2. The Expression of NMDA receptor and SP auto-receptor changes in dorsal root ganglion after capsaicin-induced pain in rats Objective: Our study was to investigate whether SP was involved in the modulation of the primary sensory information. With immunohistochemical technique, we observed the expression of SP changes in dorsal root ganglion (DRG) and the corresponding segments of spinal cord after capsaicin-induced pain in rats. Methods: To investigate these effects, 40 SD rats were randomly divided into 5 groups, Group A: normal control group; Group B: saline group; Group C: capsaicin 1 h group; Group D: capsaicin 6 h group; Group E: capsaicin 12 h group. Resp